DNAJB11 Polyklonaler Antikörper

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• KD/KO Validated

DNAJB11 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

19

Kat-Nr. : 15484-1-AP

Synonyme

ABBP 2, ABBP2, APOBEC1 binding protein 2, DJ9, DnaJ protein homolog 9, DNAJB11, EDJ, ER associated DNAJ, ER associated dnaJ protein 3, ERdj3, ERj3, ERj3p, hDj 9, hDj9, HEDJ, Human DnaJ protein 9, PRO1080, PWP1 interacting protein 4, UNQ537, UNQ537/PRO1080

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Galerie der Validierungsdaten

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  WB analysis using 15484-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15484-1-AP (DNAJB11 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15484-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15484-1-AP (DNAJB11 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 15484-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15484-1-AP (DNAJB11 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 15484-1-AP

  IP Result of anti-DNAJB11 (IP:15484-1-AP, 4ug; Detection:15484-1-AP 1:500) with mouse heart tissue lysate 3200ug.

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  IHC staining of human ovary tumor using 15484-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15484-1-AP (DNAJB11 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary tumor using 15484-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15484-1-AP (DNAJB11 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HepG2 using 15484-1-AP

  Immunofluorescent analysis of HepG2 cells, using DNAJB11 antibody 15484-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Mausherzgewebe, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 18 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Produktinformation

15484-1-AP bindet in WB, IP, IHC, IF, ELISA DNAJB11 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNAJB11 fusion protein Ag7832
• Vollständiger Name: DnaJ (Hsp40) homolog, subfamily B, member 11
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC001144
• Gene symbol: DNAJB11
• Gene ID (NCBI): 51726
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Science; The endoplasmic reticulum P5A-ATPase is a transmembrane helix dislocase.; Authors - Michael J McKenna
human	WB	Cell; Defining Hsp70 Subnetworks in Dengue Virus Replication Reveals Key Vulnerability in Flavivirus Infection.; Authors - Shuhei Taguwa ; KD Validated
mouse	WB	Mol Cell; Differentiation Drives Widespread Rewiring of the Neural Stem Cell Chaperone Network.; Authors - Willianne I M Vonk
human	WB, IP	EMBO J; Unfolded protein response-induced ERdj3 secretion links ER stress to extracellular proteostasis.; Authors - Joseph C Genereux ; KD Validated
human	WB	Cell Chem Biol; Quantitative Interactome Proteomics Reveals a Molecular Basis for ATF6-Dependent Regulation of a Destabilized Amyloidogenic Protein.; Authors - Lars Plate
human	WB	Cell Death Dis; Shutdown of ER-associated degradation pathway rescues functions of mutant iduronate 2-sulfatase linked to mucopolysaccharidosis type II.; Authors - Yosuke Osaki ; KD Validated