DNAJB12 Polyklonaler Antikörper

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• KD/KO Validated

DNAJB12 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Kat-Nr. : 16780-1-AP

Synonyme

DJ10

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  WB analysis using 16780-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16780-1-AP (DNAJB12 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16780-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16780-1-AP (DNAJB12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16780-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16780-1-AP (DNAJB12 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 16780-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16780-1-AP (DNAJB12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16780-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16780-1-AP (DNAJB12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 16780-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 16780-1-AP (DNAJB12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 16780-1-AP

  IP result of anti-DNAJB12 (IP:16780-1-AP, 4ug; Detection:16780-1-AP 1:500) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human stomach using 16780-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16780-1-AP (DNAJB12 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 16780-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16780-1-AP (DNAJB12 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 16780-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16780-1-AP (DNAJB12 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 16780-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16780-1-AP (DNAJB12 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, A549-Zellen, humanes Hirngewebe, humanes Nierengewebe, humanes Lebergewebe, MCF-7-Zellen, MKN-45-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Magengewebe, human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IF: See 3 publications below
• IP: See 3 publications below
• ChIP: See 1 publications below

Produktinformation

16780-1-AP bindet in WB, IHC, IF, IP, ChIP, ELISA DNAJB12 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNAJB12 fusion protein Ag10193
• Vollständiger Name: DnaJ (Hsp40) homolog, subfamily B, member 12
• Berechnetes Molekulargewicht: 375 aa, 42 kDa
• Beobachtetes Molekulargewicht: 40-42 kDa
• GenBank-Zugangsnummer: BC011812
• Gene symbol: DNAJB12
• Gene ID (NCBI): 54788
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNAJB12 (JB12), a ER transmembrane chaperone, specifically interacts with Hsc70 to facilitate ER retention and delivery of membrane proteins for folding or degradation in the ERAD pathway. Hsc70 chaperone activities require the substrate targeting function of DNAJB12. DNAJB12 recruits specific substrates to contact Hsc70 and then stimulates its ATPase activity. (PMID: 34668642)

Publikationen

Species	Application	Title
human	WB,ChIP	Mol Cell; An oncogenic JMJD6-DGAT1 axis tunes the epigenetic regulation of lipid droplet formation in clear cell renal cell carcinoma.; Authors - Jin Zhou
human	IP	Nat Commun; The methyltransferase METTL9 mediates pervasive 1-methylhistidine modification in mammalian proteomes.; Authors - Erna Davydova
	WB, IP	J Cell Biol; Reticulon protects the integrity of the ER membrane during ER escape of large macromolecular protein complexes.; Authors - Yu-Jie Chen
human	WB	PLoS Pathog; A cytosolic chaperone complexes with dynamic membrane J-proteins and mobilizes a nonenveloped virus out of the endoplasmic reticulum.; Authors - Christopher Paul Walczak
human	WB,IF	J Cell Biol; Reticulons promote formation of ER-derived double-membrane vesicles that facilitate SARS-CoV-2 replication; Authors - Jeffrey M Williams
human	IF	J Virol; The ER membrane J protein C18 executes a distinct role in promoting SV40 membrane penetration.; Authors - Parikshit Bagchi