DNAJB14 Polyklonaler Antikörper

DNAJB14 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 16501-1-AP

Synonyme

UNQ9427/PRO34683, PRO34683, EGNR9427

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  WB analysis of Jurkat using 16501-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16501-1-AP (DNAJB14 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16501-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16501-1-AP (DNAJB14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 16501-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 16501-1-AP (DNAJB14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 16501-1-AP

  IP result of anti-DNAJB14 (IP:16501-1-AP, 4ug; Detection:16501-1-AP 1:1000) with Jurkat cells lysate 3080ug.

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  IHC staining of human ovary cancer using 16501-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16501-1-AP (DNAJB14 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 16501-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DNAJB14 antibody (16501-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, humanes Hodengewebe
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IF: See 3 publications below

Produktinformation

16501-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA DNAJB14 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNAJB14 fusion protein Ag9668
• Vollständiger Name: DnaJ (Hsp40) homolog, subfamily B, member 14
• Berechnetes Molekulargewicht: 379 aa, 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC022248
• Gene symbol: DNAJB14
• Gene ID (NCBI): 79982
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IF	Nat Commun; Selective EMC subunits act as molecular tethers of intracellular organelles exploited during viral entry.; Authors - Parikshit Bagchi
human	WB	PLoS Pathog; A cytosolic chaperone complexes with dynamic membrane J-proteins and mobilizes a nonenveloped virus out of the endoplasmic reticulum.; Authors - Christopher Paul Walczak
	WB	PLoS Pathog; A Non-enveloped Virus Hijacks Host Disaggregation Machinery to Translocate across the Endoplasmic Reticulum Membrane.; Authors - Madhu Sudhan Ravindran
human	IF	J Virol; The ER membrane J protein C18 executes a distinct role in promoting SV40 membrane penetration.; Authors - Parikshit Bagchi
human	WB	J Virol; SGTA-dependent regulation of Hsc70 promotes cytosol entry of SV40 from the ER.; Authors - Allison Dupzyk
human	WB	J Virol; Ubqln4 facilitates ER-to-cytosol escape of a nonenveloped virus during infection.; Authors - Xiaofang Liu