DNAJB14 Polyklonaler Antikörper

DNAJB14 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16501-1-PBS

Synonyme

UNQ9427/PRO34683, PRO34683, EGNR9427

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  WB analysis of Jurkat using 16501-1-AP (same clone as 16501-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16501-1-AP (DNAJB14 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16501-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 16501-1-AP (same clone as 16501-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16501-1-AP (DNAJB14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16501-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 16501-1-AP (same clone as 16501-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 16501-1-AP (DNAJB14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16501-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 16501-1-AP (same clone as 16501-1-PBS)

  IP result of anti-DNAJB14 (IP:16501-1-AP, 4ug; Detection:16501-1-AP 1:1000) with Jurkat cells lysate 3080ug. This data was developed using the same antibody clone with 16501-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 16501-1-AP (same clone as 16501-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16501-1-AP (DNAJB14 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16501-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 16501-1-AP (same clone as 16501-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DNAJB14 antibody (16501-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 16501-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16501-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA DNAJB14 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNAJB14 fusion protein Ag9668
• Vollständiger Name: DnaJ (Hsp40) homolog, subfamily B, member 14
• Berechnetes Molekulargewicht: 379 aa, 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC022248
• Gene symbol: DNAJB14
• Gene ID (NCBI): 79982
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.