DNAJB2 Polyklonaler Antikörper

DNAJB2 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 10838-1-AP

Synonyme

HSJ-1, HSJ1, HSJ 1, Heat shock protein J1, Heat shock 40 kDa protein 3

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  WB analysis using 10838-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10838-1-AP (DNAJB2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10838-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10838-1-AP (DNAJB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 10838-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10838-1-AP (DNAJB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10838-1-AP

  IP result of anti-DNAJB2 (IP:10838-1-AP, 4ug; Detection:10838-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human stomach using 10838-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10838-1-AP (DNAJB2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, A431-Zellen, Maushirngewebe, Maus-Skelettmuskelgewebe, Rattenhirngewebe, Rattenherzgewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Produktinformation

10838-1-AP bindet in WB, IHC, IF, IP, ELISA DNAJB2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNAJB2 fusion protein Ag1271
• Vollständiger Name: DnaJ (Hsp40) homolog, subfamily B, member 2
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 36-40 kDa
• GenBank-Zugangsnummer: BC011609
• Gene symbol: DNAJB2
• Gene ID (NCBI): 3300
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The DNAJB2 (also known as HSJ1a, Heat-Shock Protein J1a) is a member of the DNAJ family of molecular chaperones which play important cellular roles in correct protein folding, in the response to protein misfolding, and in the degradation of misfolded proteins. It is expressed principally in the nervous system and is a key player in neuronal proteostasis maintenance. DNAJB2 has been proposed as a potent anti-aggregation chaperone for TDP-43, may play a role in the pathogenesis of ALS. DNAJB2 gene encodes two alternatively-spliced isoforms that differ in their C-terminus. The variant 1 form (V1 or HSJ1a) is expressed in both cytoplasm and nucleus, whereas the variant 2 form (V2 or HSJ1b) undergoes post-translational geranylgeranylation modification which mediates its attachment to the cytoplasmic side of the endoplasmic reticulum membrane. This antibody detects both isoforms of DNAJB2 with MW between 32-40 kDa. (PMID: 20395441)

Publikationen

Species	Application	Title
human	WB, IF	Ann Neurol; A rare recessive distal hereditary motor neuropathy with HSJ1 chaperone mutation.; Authors - Blumen Sergiu C SC
		Diabetes; PMID: 24379352; Authors - Brian G Drew
human,mouse	WB,IHC	Am J Pathol; DNAJB2 expression in normal and diseased human and mouse skeletal muscle.; Authors - Claeys Kristl G KG
human	WB,IHC	Eur J Neurol; DNAJB2-related Charcot-Marie-Tooth disease type 2: Pathomechanism insights and phenotypic spectrum widening.; Authors - Paola Saveri
human	WB,IF	Sci Rep; PolyQ-expanded proteins impair cellular proteostasis of ataxin-3 through sequestering the co-chaperone HSJ1 into aggregates.; Authors - Hong-Wei Yue
human	WB	Cancers (Basel); Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin; Authors - Mohamed Alalem