DNAJB2 Polyklonaler Antikörper

DNAJB2 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 10838-1-AP

Synonyme

HSJ-1, HSJ1, HSJ 1, Heat shock protein J1, Heat shock 40 kDa protein 3



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMausherzgewebe, A431-Zellen, Maushirngewebe, Maus-Skelettmuskelgewebe, Rattenhirngewebe, Rattenherzgewebe
Erfolgreiche IPMaushirngewebe
Erfolgreiche Detektion in IHChumanes Magengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:5000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

10838-1-AP bindet in WB, IHC, IF, IP, ELISA DNAJB2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen DNAJB2 fusion protein Ag1271
Vollständiger Name DnaJ (Hsp40) homolog, subfamily B, member 2
Berechnetes Molekulargewicht 36 kDa
Beobachtetes Molekulargewicht 36-40 kDa
GenBank-ZugangsnummerBC011609
Gene symbol DNAJB2
Gene ID (NCBI) 3300
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The DNAJB2 (also known as HSJ1a, Heat-Shock Protein J1a) is a member of the DNAJ family of molecular chaperones which play important cellular roles in correct protein folding, in the response to protein misfolding, and in the degradation of misfolded proteins. It is expressed principally in the nervous system and is a key player in neuronal proteostasis maintenance. DNAJB2 has been proposed as a potent anti-aggregation chaperone for TDP-43, may play a role in the pathogenesis of ALS. DNAJB2 gene encodes two alternatively-spliced isoforms that differ in their C-terminus. The variant 1 form (V1 or HSJ1a) is expressed in both cytoplasm and nucleus, whereas the variant 2 form (V2 or HSJ1b) undergoes post-translational geranylgeranylation modification which mediates its attachment to the cytoplasmic side of the endoplasmic reticulum membrane. This antibody detects both isoforms of DNAJB2 with MW between 32-40 kDa. (PMID: 20395441)

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for DNAJB2 antibody 10838-1-APProtokoll herunterladen
IHC protocol for DNAJB2 antibody 10838-1-APProtokoll herunterladenl
IP protocol for DNAJB2 antibody 10838-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB, IF

Ann Neurol

A rare recessive distal hereditary motor neuropathy with HSJ1 chaperone mutation.

Authors - Blumen Sergiu C SC

Diabetes

PMID: 24379352

Authors - Brian G Drew
human,mouseWB,IHC

Am J Pathol

DNAJB2 expression in normal and diseased human and mouse skeletal muscle.

Authors - Claeys Kristl G KG
humanWB,IHC

Eur J Neurol

DNAJB2-related Charcot-Marie-Tooth disease type 2: Pathomechanism insights and phenotypic spectrum widening.

Authors - Paola Saveri
humanWB,IF

Sci Rep

PolyQ-expanded proteins impair cellular proteostasis of ataxin-3 through sequestering the co-chaperone HSJ1 into aggregates.

Authors - Hong-Wei Yue
humanWB

Cancers (Basel)

Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin

Authors - Mohamed Alalem