DNAJB3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DNAJB3 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 17177-1-AP

Synonyme

DNAJB3, HCG3

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse testis using 17177-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17177-1-AP (DNAJB3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17177-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17177-1-AP (DNAJB3 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17177-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17177-1-AP (DNAJB3 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 17177-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 17177-1-AP (DNAJB3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 17177-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17177-1-AP (DNAJB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 17177-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17177-1-AP (DNAJB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 17177-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17177-1-AP (DNAJB3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17177-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17177-1-AP (DNAJB3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17177-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17177-1-AP (DNAJB3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17177-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17177-1-AP (DNAJB3 antibody) at dilution of 1:100 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Eierstockgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 2 publications below

Produktinformation

17177-1-AP bindet in WB, IHC, ELISA DNAJB3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNAJB3 fusion protein Ag10878
• Vollständiger Name: DnaJ (Hsp40) homolog, subfamily B, member 3
• Berechnetes Molekulargewicht: 145 aa, 17 kDa
• Beobachtetes Molekulargewicht: 28-30 kDa
• GenBank-Zugangsnummer: BC024013
• Gene symbol: DNAJB3
• Gene ID (NCBI): 414061
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Sci Rep; PMID: 26400768; Authors - Mohamed Abu-Farha
mouse	WB	Sci Rep; Alpha lipoic acid attenuates ER stress and improves glucose uptake through DNAJB3 cochaperone.; Authors - Abdoulaye Diane ; KD Validated
human	WB,IHC	PLoS One; DNAJB3/HSP-40 Cochaperone Is Downregulated in Obese Humans and Is Restored by Physical Exercise.; Authors - Abubaker Jehad J
mouse	WB	Int J Mol Sci; Expression of Human DNAJ (Heat Shock Protein-40) B3 in Humanized UDP-glucuronosyltransferase 1 Mice.; Authors - Ryo Mitsugi
human	WB,IHC	Lipids Health Dis; Immunohistochemical profiling of the heat shock response in obese non-diabetic subjects revealed impaired expression of heat shock proteins in the adipose tissue.; Authors - Ali Tiss
mouse	WB	Sci Rep; DNAJB3 attenuates metabolic stress and promotes glucose uptake by eliciting Glut4 translocation.; Authors - Abdelilah Arredouani ; KD Validated