DNAJB5 Polyklonaler Antikörper

DNAJB5 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16453-1-AP

Synonyme

KIAA1045, Hsc40, Heat shock protein Hsp40-3, Heat shock protein Hsp40-2, Heat shock protein Hsp40 3

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  WB analysis of rat heart using 16453-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 16453-1-AP (DNAJB5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16453-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16453-1-AP (DNAJB5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16453-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16453-1-AP (DNAJB5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 16453-1-AP

  IP result of anti-DNAJB5 (IP:16453-1-AP, 4ug; Detection:16453-1-AP 1:500) with HepG2 cells lysate 3300 ug.

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  IHC staining of human heart using 16453-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 16453-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 16453-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 16453-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 16453-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 16453-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 16453-1-AP

  Immunofluorescent analysis of HepG2 cells using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenherzgewebe, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Nierengewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16453-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA DNAJB5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNAJB5 fusion protein Ag9341
• Vollständiger Name: DnaJ (Hsp40) homolog, subfamily B, member 5
• Berechnetes Molekulargewicht: 386 aa, 44 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC012115
• Gene symbol: DNAJB5
• Gene ID (NCBI): 25822
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNAJB5, also named as Hsc40, is an Hsp40 Family Protein with MW 39-40 kDa. It mediates antihypertrophic effects of Thioredoxin1 in the heart. This protein has some isoforms with MW 39 kDa and 47-51 kDa.