DNAJB6 Monoklonaler Antikörper

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• KD/KO Validated

DNAJB6 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

CloneNo.

1A1D2

Kat-Nr. : 66587-1-Ig

Synonyme

DJ4, DnaJ, Heat shock protein J2, HHDJ1, HSJ 2

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  WB analysis of HEK-293 using 66587-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66587-1-Ig (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66587-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66587-1-Ig (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 66587-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66587-1-Ig (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66587-1-Ig

  WB result of DNAJB6 antibody (66587-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJB6 transfected HeLa cells.

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  WB analysis of HSC-T6 using 66587-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66587-1-Ig (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 66587-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66587-1-Ig (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 66587-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66587-1-Ig (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66587-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66587-1-Ig (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 66587-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66587-1-Ig (DNAJB6 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 66587-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66587-1-Ig (DNAJB6 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66587-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66587-1-Ig (DNAJB6 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66587-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66587-1-Ig (DNAJB6 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66587-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66587-1-Ig (DNAJB6 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66587-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66587-1-Ig (DNAJB6 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66587-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DNAJB6 antibody (66587-1-Ig, Clone: 1A1D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen, HeLa-Zellen, Maushirngewebe, Neuro-2a-Zellen, Hausschwein-Hirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:8000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

66587-1-Ig bindet in WB, IHC, IF/ICC, ELISA DNAJB6 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DNAJB6 fusion protein Ag2258
• Vollständiger Name: DnaJ (Hsp40) homolog, subfamily B, member 6
• Berechnetes Molekulargewicht: 241 aa, 27 kDa
• Beobachtetes Molekulargewicht: 27 kDa, 38 kDa
• GenBank-Zugangsnummer: BC000177
• Gene symbol: DNAJB6
• Gene ID (NCBI): 10049
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNAJB6, belongs to the heat shock protein 40 (HSP40) family, a noncanonical member of the DNAJ-chaperone family, is mainly expressed in neurons. It plays an important role in recovery from misfolded protein aggregates (PMID: 27276715). As seen by the western blot stained for DNAJB6, there are 2 splice forms of the protein resulting in a longer isoform (38 kDa) and a shorter one (27 kDa). DNAJB6 (27 kDa) isoform but not DNAJB6 (38 kDa) isoform is effective with suppressing cytoplasmic protein aggregation (PMID: 20159555). The antibody 66587-1-Ig recognizes both isoforms.

Publikationen

Species	Application	Title
human	IHC	Transl Oncol; Development of novel DNAJB6-KIAA1522-p-mTOR three-protein prognostic prediction models for CRC; Authors - Yu-Juan Jiang
human	WB	Int J Biol Macromol; DnaJs are enriched in tau regulators; Authors - Abigail R Esquivel ; KD Validated
human	IF	Glia; DNAJB6 is expressed in neurons and oligodendrocytes of the human brain; Authors - Jónvá Hentze
human	WB,IHC	Biomedicines; Abnormally High Expression of DNAJB6 Accelerates Malignant Progression of Lung Adenocarcinoma; Authors - Di Wang ; KD Validated