DNAJB9 Polyklonaler Antikörper

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• KD/KO Validated

DNAJB9 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 13157-1-AP

Synonyme

DNAJB9, ERdj4, Mdg 1, MDG1, MST049, MSTP049

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  WB analysis using 13157-1-AP

  Mouse kidney lysates were subjected to SDS PAGE followed by western blot with 13157-1-AP (DNAJB9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 13157-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13157-1-AP (DNAJB9 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  IP experiment of mouse testis using 13157-1-AP

  IP result of anti-DNAJB9 (IP:13157-1-AP, 4ug; Detection:13157-1-AP 1:500) with mouse testis tissue lysate 4800ug.

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  IHC staining of human breast cancer using 13157-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13157-1-AP (DNAJB9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13157-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13157-1-AP (DNAJB9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 13157-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13157-1-AP (DNAJB9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 13157-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13157-1-AP (DNAJB9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, Maushodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below

Produktinformation

13157-1-AP bindet in WB, IP, IHC, ELISA DNAJB9 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNAJB9 fusion protein Ag3789
• Vollständiger Name: DnaJ (Hsp40) homolog, subfamily B, member 9
• Berechnetes Molekulargewicht: 223 aa, 26 kDa
• Beobachtetes Molekulargewicht: 26-30 kDa
• GenBank-Zugangsnummer: BC028912
• Gene symbol: DNAJB9
• Gene ID (NCBI): 4189
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human,mouse	WB	J Pineal Res; Melatonin alleviates PM2.5 -induced glucose metabolism disorder and lipidome alteration by regulating endoplasmic reticulum stress; Authors - Zhou Du
human	WB	Mol Cell; Coordination between Two Branches of the Unfolded Protein Response Determines Apoptotic Cell Fate.; Authors - Tsun-Kai Chang
human	WB	Elife; Cell-based HTS identifies a chemical chaperone for preventing ER protein aggregation and proteotoxicity.; Authors - Keisuke Kitakaze
human	WB	Hum Mol Genet; XBP1 variant 1 (Xv1) promotes mitosis of cancer cells involving upregulation of the polyglutamylase TTLL6.; Authors - Yongwang Zhong
human	IHC	Pathol Int; Early onset Congo red-positive fibrillary glomerulonephritis associated with glomerular DNAJB9 deposits mimicking renal amyloidosis; Authors - Mizue Goto
human	IHC	Case Rep Nephrol; Fibrillary Glomerulopathy with a High Level of Myeloperoxidase-ANCA: A Case Report.; Authors - Tomohiko Asakawa