DNAJC10 Polyklonaler Antikörper

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• KD/KO Validated

DNAJC10 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 13101-1-AP

Synonyme

DNAJC10, ER resident protein ERdj5, ERdj5, JPDI, Macrothioredoxin, MTHr

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 13101-1-AP

  WB result of DNAJC10 antibody (13101-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJC10 transfected HeLa cells.

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  WB analysis using 13101-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13101-1-AP (DNAJC10 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 13101-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13101-1-AP (DNAJC10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13101-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13101-1-AP (DNAJC10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13101-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13101-1-AP (DNAJC10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13101-1-AP

  IP result of anti-DNAJC10 (IP:13101-1-AP, 4ug; Detection:13101-1-AP 1:500) with HeLa cells lysate 1600 ug.

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  IHC staining of human pancreas cancer using 13101-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13101-1-AP (DNAJC10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 13101-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13101-1-AP (DNAJC10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 13101-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13101-1-AP (DNAJC10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 13101-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13101-1-AP (DNAJC10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 13101-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 13101-1-AP (DNAJC10 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach using 13101-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 13101-1-AP (DNAJC10 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 13101-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13101-1-AP (DNAJC10 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 13101-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13101-1-AP (DNAJC10 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 13101-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13101-1-AP (DNAJC10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Mauslebergewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Hodengewebe, humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

13101-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA DNAJC10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNAJC10 fusion protein Ag3737
• Vollständiger Name: DnaJ (Hsp40) homolog, subfamily C, member 10
• Berechnetes Molekulargewicht: 793 aa, 91 kDa
• Beobachtetes Molekulargewicht: 80-90 kDa
• GenBank-Zugangsnummer: BC034713
• Gene symbol: DNAJC10
• Gene ID (NCBI): 54431
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNAJC10(DnaJ homolog subfamily C member 10)is also named as ERDJ5. The human ER-resident protein (ERdj5) ubiquitously expresses and is abundant in secretory tissues such as pancreas and testis and it may be involved in assisting protein folding and quality control in the ER(PMID:12411443). The deduced 793-amino acid protein has a calculated molecular mass of about 91 kD. ERDJ5 contains an N-terminal hydrophobic sequence, followed by a type III DnaJ domain, 4 thioredoxin-like domains, and a C-terminal KDEL endoplasmic reticulum (ER) retention signal. It can be modified by N-linked glycosylation(PMID:12446677). The ERdj5 antibody displays a higher affinity for endogenous ERdj5u.

Publikationen

Species	Application	Title
human	WB	PLoS Pathog; A cytosolic chaperone complexes with dynamic membrane J-proteins and mobilizes a nonenveloped virus out of the endoplasmic reticulum.; Authors - Christopher Paul Walczak
human	WB,CoIP	Mol Cell Proteomics; Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis; Authors - Madison T. Wright
human	WB	Diabetes; Unbiased Profiling of the Human Proinsulin Biosynthetic Interaction Network Reveals a Role For Peroxiredoxin 4 in Proinsulin Folding.; Authors - Duc T Tran
human	WB	Mol Cell Proteomics; Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis.; Authors - Madison T Wright
human	WB	Mol Biol Cell; The ERdj5-Sel1L complex facilitates cholera toxin retrotranslocation.; Authors - Williams Jeffrey M JM
human	WB	Cell Stress Chaperones; Amyotrophic lateral sclerosis (ALS) and Alzheimer's disease (AD) are characterised by differential activation of ER stress pathways: focus on UPR target genes.; Authors - L Montibeller