DNAJC11 Polyklonaler Antikörper

DNAJC11 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 17331-1-AP

Synonyme

DNAJC11

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 17331-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17331-1-AP (DNAJC11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17331-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17331-1-AP (DNAJC11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17331-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17331-1-AP (DNAJC11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 17331-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17331-1-AP (DNAJC11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 17331-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17331-1-AP (DNAJC11 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 17331-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17331-1-AP (DNAJC11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 17331-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17331-1-AP (DNAJC11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17331-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17331-1-AP (DNAJC11 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse brain using 17331-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17331-1-AP (DNAJC11 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HepG2-Zellen, Mauslebergewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Skelettmuskelgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

17331-1-AP bindet in WB, IHC, ELISA DNAJC11 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNAJC11 fusion protein Ag11310
• Vollständiger Name: DnaJ (Hsp40) homolog, subfamily C, member 11
• Berechnetes Molekulargewicht: 507 aa, 57 kDa
• Beobachtetes Molekulargewicht: 63 kDa
• GenBank-Zugangsnummer: BC014145
• Gene symbol: DNAJC11
• Gene ID (NCBI): 55735
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNAJC11 is a member of the J proteins also known as HSP40 family of co-chaperones. Recently DNAJC11 has been found to interact with mitofilin, a mitochondrial inner membrane protein, indicating that it may be a molecular chaperone during mitochondrial protein import and folding.

Publikationen

Species	Application	Title
human	WB	J Proteome Res; Mapping Interactome Networks of DNAJC11, a Novel Mitochondrial Protein Causing Neuromuscular Pathology in Mice.; Authors - Foteini Violitzi
mouse	WB	PLoS One; A Splicing Mutation in the Novel Mitochondrial Protein DNAJC11 Causes Motor Neuron Pathology Associated with Cristae Disorganization, and Lymphoid Abnormalities in Mice.; Authors - Fotis Ioakeimidis
human,mouse	WB	Nat Cell Biol; Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space; Authors - Elias Adriaenssens