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DNAJC13 Polyklonaler Antikörper

DNAJC13 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31492-1-PBS

Synonyme

RME8, RME 8, KIAA0678

Filter:
  • Western Blot (WB) analysis of various lysates using DNAJC13 Polyclonal antibody (31492-1-AP)

    WB analysis using 31492-1-AP (same clone as 31492-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31492-1-AP (DNAJC13 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31492-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DNAJC13 Polyclonal antibody (31492-1-AP)

    IF Staining of HeLa using 31492-1-AP (same clone as 31492-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DNAJC13 antibody (31492-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 31492-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

31492-1-PBS bindet in WB, IF/ICC, Indirect ELISA DNAJC13 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen DNAJC13 fusion protein Ag35412
Vollständiger Name DnaJ (Hsp40) homolog, subfamily C, member 13
Beobachtetes Molekulargewicht254 kDa
GenBank-ZugangsnummerBC043583
Gene symbol DNAJC13
Gene ID (NCBI) 23317
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNAJC13 is a large protein with 2,243 amino acids. Due to the presence of the central DNAJ domain, DNAJC13 binds HSC70 (heat shock cognate 70) and acts as a co-chaperone in mediating HSC70 cellular functions. Within the cell, DNAJC13 is primarily localized at membranous structures. This interaction is mediated, at least in part, by the binding to phosphoinositides (PI) through its N-terminal sequence. RME-8/DNAJC13 is involved in several endosomal functions such as protein sorting, endosomal tubulation, transport processes including the retrograde transport from the endosome to the trans-Golgi network (TGN), the formation of endosomal degradative microdomains, and the recycling of membrane receptors. (PMID: 32322926)