DNAJC19 Polyklonaler Antikörper

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• KD/KO Validated

DNAJC19 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12096-1-PBS

Synonyme

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  WB analysis of HeLa using 12096-1-AP (same clone as 12096-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12096-1-AP (DNAJC19 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12096-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 12096-1-AP (same clone as 12096-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12096-1-AP (DNAJC19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12096-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 12096-1-AP (same clone as 12096-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12096-1-AP (DNAJC19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12096-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human lung using 12096-1-AP (same clone as 12096-1-PBS)

  human lung tissue were subjected to SDS PAGE followed by western blot with 12096-1-AP (DNAJC19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12096-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse heart using 12096-1-AP (same clone as 12096-1-PBS)

  IP result of anti-DNAJC19 (IP:12096-1-AP, 4ug; Detection:12096-1-AP 1:1000) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 12096-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 12096-1-AP (same clone as 12096-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DNAJC19 antibody (12096-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12096-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12096-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA DNAJC19 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNAJC19 fusion protein Ag2739
• Vollständiger Name: DnaJ (Hsp40) homolog, subfamily C, member 19
• Berechnetes Molekulargewicht: 116 aa, 13 kDa
• Beobachtetes Molekulargewicht: 13 kDa
• GenBank-Zugangsnummer: BC009702
• Gene symbol: DNAJC19
• Gene ID (NCBI): 131118
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNAJC19 is a mitochondrial cochaperone that interacts with HSP70 chaperones through its conserved J-domain. As a transmembrane protein, DNAJC19 is strongly associated with the inner mitochondrial membrane. Mutations in DNAJC19 cause dilated cardiomyopathy with ataxia (DCMA). Recently DNAJC19 has been reported as interactor of PHB complex to regulate cardiolipin remodeling, which addressed the link of DNAJC19 to cardiomyopathy. This antibody is specific to DNAJC19 and had been tested by siRNA.(24856930)