DNAJC21 Polyklonaler Antikörper

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• KD/KO Validated

DNAJC21 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 23411-1-AP

Synonyme

DNAJA5, DNAJC21, GS3, JJJ1, Protein GS3

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 23411-1-AP

  WB results of DNAJC21 antibody (23411-1-AP, 1:2000) with si-control and si-DNAJC21 transfected HEK293 cells.

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  WB analysis using 23411-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23411-1-AP (DNAJC21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 23411-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23411-1-AP (DNAJC21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 23411-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23411-1-AP (DNAJC21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 23411-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 23411-1-AP (DNAJC21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 23411-1-AP

  IP Result of anti-DNAJC21 (IP:23411-1-AP, 4ug; Detection:23411-1-AP 1:1000) with HEK-293 cells lysate 2700ug.

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  IHC staining of human pancreas using 23411-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 23411-1-AP (DNAJC21 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 23411-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 23411-1-AP (DNAJC21 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 23411-1-AP

  Immunofluorescent analysis of HeLa cells using 23411-1-AP (DNAJC21 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

23411-1-AP bindet in WB, IP, IHC, IF, ELISA DNAJC21 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNAJC21 fusion protein Ag20003
• Vollständiger Name: DnaJ (Hsp40) homolog, subfamily C, member 21
• Berechnetes Molekulargewicht: 531 aa, 58 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC107577
• Gene symbol: DNAJC21
• Gene ID (NCBI): 134218
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNAJC21 is a ubiquitously expressed DnaJ (heat shock protein 40) chaperone protein. Recently DNAJC21 was reported to be implicated in rRNA biogenesis. Loss of DNAJC21 induced abnormal cell morphology and inhibited cell growth. Mutations in DNAJC21 cause bone marrow failure syndrome. (27346687)

Publikationen

Species	Application	Title
human	WB	Cell Stem Cell; HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis.; Authors - Kaosheng Lv
human	WB,IF	Am J Hum Genet; DNAJC21 Mutations Link a Cancer-Prone Bone Marrow Failure Syndrome to Corruption in 60S Ribosome Subunit Maturation.; Authors - Hemanth Tummala ; KD Validated