DNAJC6/AUXILIN Polyklonaler Antikörper

DNAJC6/AUXILIN Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 21941-1-AP

Synonyme

AUXILIN, DJC6, DNAJC6, DNAJC6/AUXILIN, KIAA0473

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 21941-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21941-1-AP (DNAJC6/AUXILIN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 21941-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21941-1-AP (DNAJC6/AUXILIN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 21941-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21941-1-AP (DNAJC6/AUXILIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 21941-1-AP

  IP result of anti-DNAJC6/AUXILIN (IP:21941-1-AP, 4ug; Detection:21941-1-AP 1:2000) with mouse brain tissue lysate 4000 ug.

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  IHC staining of human brain using 21941-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21941-1-AP (DNAJC6/AUXILIN antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 21941-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21941-1-AP (DNAJC6/AUXILIN antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 21941-1-AP

  Immunofluorescent analysis of HeLa cells using 21941-1-AP (DNAJC6/AUXILIN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen, humanes Hirngewebe, Maus-Cerebellum-Gewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

21941-1-AP bindet in WB, IP, IHC, IF, ELISA DNAJC6/AUXILIN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNAJC6/AUXILIN fusion protein Ag16612
• Vollständiger Name: DnaJ (Hsp40) homolog, subfamily C, member 6
• Berechnetes Molekulargewicht: 970 aa, 106 kDa
• Beobachtetes Molekulargewicht: 100-120 kDa, 86 kDa
• GenBank-Zugangsnummer: BC109279
• Gene symbol: DNAJC6
• Gene ID (NCBI): 9829
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNAJC6, also named AUXILIN, belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. It is a neuronal protein that functions specifically in the pathway of clathrin-mediated endocytosis. It shares homology with GAK, and the two proteins act as cochaperones to support the HSC70 -dependent clathrin uncoating of clathrin-coated vesicles (PMID: 2016009). DNAJC6 is expressed in various brain regions, including cerebellum, corpus callosum, cortex, striatum, brainstem, pons, putamen, spinal cord and substantia nigra (PMID: 23211418).

Publikationen

Species	Application	Title
mouse		Sci Rep; Differential regulation of synaptic AP-2/clathrin vesicle uncoating in synaptic plasticity.; Authors - Ermes Candiello
human	WB	Cancers (Basel); Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin; Authors - Mohamed Alalem