DNAJC7 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DNAJC7 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 11090-1-AP

Synonyme

DANJC7, DJ11, DNAJC7, TPR repeat protein 2, TPR2, TTC2

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Galerie der Validierungsdaten

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  WB analysis using 11090-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11090-1-AP (DNAJC7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 11090-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11090-1-AP (DNAJC7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 11090-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11090-1-AP (DNAJC7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11090-1-AP

  Immunofluorescent analysis of HepG2 cells, using DNAJC7 antibody 11090-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HepG2 using 11090-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug DNAJC7 antibody (11090-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

11090-1-AP bindet in WB, IHC, IF, FC, CoIP, ELISA DNAJC7 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNAJC7 fusion protein Ag1572
• Vollständiger Name: DnaJ (Hsp40) homolog, subfamily C, member 7
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC011837
• Gene symbol: DNAJC7
• Gene ID (NCBI): 7266
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DnaJC7, otherwise referred to as Tpr2 or CCRP, is a conserved, type of J protein that is highly expressed within the brain. DnaJC7 has been found to modulate the flow of substrates from Hsp70 to Hsp90, providing a control mechanism in proteins such as glucocorticoids and progesterone receptor.

Publikationen

Species	Application	Title
mouse	CoIP	Nat Commun; DnaJC7 binds natively folded structural elements in tau to inhibit amyloid formation.; Authors - Zhiqiang Hou
human	WB,IF	Biochem Pharmacol; Indirect activation of constitutive androstane receptor in three-dimensionally cultured HepG2 cells.; Authors - Kosuke Yokobori ; KO Validated
human	WB	Biochem Biophys Res Commun; Co-chaperon DnaJC7/TPR2 enhances p53 stability and activity through blocking the complex formation between p53 and MDM2.; Authors - Kubo Natsumi N
human	WB	bioRxiv; DnaJC7 specifically regulates tau seeding; Authors - Valerie A Perez ; KO Validated
human	WB	Elife; DnaJC7 specifically regulates tau seeding; Authors - Valerie Ann Perez
human	WB	Cancers (Basel); Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin; Authors - Mohamed Alalem