DNAJC9 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DNAJC9 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Rezensionen (2)

Kat-Nr. : 25444-1-AP

Synonyme

SB73, KIAA0974, JDD1, HDJC9, DnaJ protein SB73

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  WB analysis of HEK-293 using 25444-1-AP

  WB result of DNAJC9 antibody (25444-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJC9 transfected HEK-293 cells.

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  WB analysis using 25444-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25444-1-AP (DNAJC9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 25444-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 25444-1-AP (DNAJC9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 25444-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25444-1-AP (DNAJC9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 25444-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25444-1-AP (DNAJC9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 25444-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25444-1-AP (DNAJC9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 25444-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25444-1-AP (DNAJC9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 25444-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 25444-1-AP (DNAJC9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 25444-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 25444-1-AP (DNAJC9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat colon using 25444-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 25444-1-AP (DNAJC9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 25444-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 25444-1-AP (DNAJC9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 25444-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25444-1-AP (DNAJC9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 25444-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25444-1-AP (DNAJC9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 25444-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DNAJC9 antibody (25444-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Raji-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe, Maus-Kolongewebe, Ratten-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

25444-1-AP bindet in WB, IHC, IF/ICC, ELISA DNAJC9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNAJC9 fusion protein Ag22123
• Vollständiger Name: DnaJ (Hsp40) homolog, subfamily C, member 9
• Berechnetes Molekulargewicht: 260 aa, 30 kDa
• Beobachtetes Molekulargewicht: 30-35 kDa
• GenBank-Zugangsnummer: BC136507
• Gene symbol: DNAJC9
• Gene ID (NCBI): 23234
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNAJC9, DnaJ homolog subfamily C member 9, acts as a dual histone chaperone and heat shock co-chaperone (PMID: 33857403). As a histone chaperone, DNAJC9 forms a co-chaperone complex with MCM2 and histone H3-H4 heterodimers (PMID: 33857403). DNAJC9 also plays a role as co-chaperone of the HSP70 family of molecular chaperone proteins. (PMID: 17182002, PMID: 33857403). DNAJC9 exhibits activity to assemble histones onto DNA (PMID: 33857403). DNAJC9 is an essential protein in many cancer cell types and the levels of the protein correlate with the rates at which cancer cells proliferate.

Publikationen

Species	Application	Title
human	WB	Sci Rep; Label-free Quantitative Analysis of Protein Expression Alterations in miR-26a-Knockout HeLa Cells using SWATH-MS Technology.; Authors - Hexiao Shen
human	WB	bioRxiv; Mechanism of ASF1 Inhibition by CDAN1; Authors - Samantha F Sedor
human	WB	Nat Commun; Mechanism of ASF1 engagement by CDAN1; Authors - Samantha F Sedor
human	WB	J Med Virol; DNAJC9 Binds to and Enhances the Transcription of Hepatitis B Virus cccDNA by Recruiting Histone H3.3; Authors - Tianhao Mao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

HELENA (Verified Customer) (04-26-2023)

Samples correspond to activated human primary CD4+ T cells after knock-out of DNAJC9 by CRISPR/CAS9.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human primary CD4+ T cells

DNAJC9 Antibody Western Blot validation (1:1000 dilution) in Human primary CD4+ T cells (Cat no:25444-1-AP)

FH

Sven (Verified Customer) (11-27-2021)

Works very well on western blot for human and mouse tissue (1:5000 dilution in TBS-T with 50% glycerol, 2% BSA, 0.02% NaN3). Antiboydy specificity was validated by DNAJC9 knockout cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Human + Mouse pancreatic cancer cell lines

DNAJC9 Antibody Western Blot validation (1:5000 dilution) in Human + Mouse pancreatic cancer cell lines (Cat no:25444-1-AP)