DNAL1 Polyklonaler Antikörper

DNAL1 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15809-1-AP

Synonyme

C14orf168, DNAL1, Dynein light chain 1, axonemal

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Galerie der Validierungsdaten

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  WB analysis using 15809-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15809-1-AP (DNAL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15809-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15809-1-AP (DNAL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15809-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15809-1-AP (DNAL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15809-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15809-1-AP (DNAL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15809-1-AP

  IP result of anti-DNAL1 (IP:15809-1-AP, 4ug; Detection:15809-1-AP 1:500) with HeLa cells lysate 2600 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hirngewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15809-1-AP bindet in WB, IP, ELISA DNAL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNAL1 fusion protein Ag8545
• Vollständiger Name: dynein, axonemal, light chain 1
• Berechnetes Molekulargewicht: 151 aa, 17 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC005343
• Gene symbol: DNAL1
• Gene ID (NCBI): 83544
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNAL1 was originally documented as the Chlamydomonas outer arm dynein axonemal light chain 1. DNAL1 has been documented to link components of the cytoskeleton with the molecular motor dynein, perhaps acting as a scaffold for larger functional structures (PMID: 22018492).