DNALI1 Polyklonaler Antikörper

DNALI1 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 17601-1-AP

Synonyme

DNALI1, hp28, P28

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Galerie der Validierungsdaten

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  WB analysis using 17601-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17601-1-AP (DNALI1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 17601-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17601-1-AP (DNALI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 17601-1-AP

  IP Result of anti-DNALI1 (IP:17601-1-AP, 4ug; Detection:17601-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, Rattenhodengewebe
• Erfolgreiche IP: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Produktinformation

17601-1-AP bindet in WB, IP, IF, CoIP, ELISA DNALI1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNALI1 fusion protein Ag11652
• Vollständiger Name: dynein, axonemal, light intermediate chain 1
• Berechnetes Molekulargewicht: 258 aa, 30 kDa
• Beobachtetes Molekulargewicht: 30-33 kDa
• GenBank-Zugangsnummer: BC046117
• Gene symbol: DNALI1
• Gene ID (NCBI): 7802
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNALI1 belongs to the inner dynein arm light chain family and encodes a subunit of the light intermediate chain of inner dynein arms of the axoneme of sperm flagella (PMID: 36792588). DNALI1 promotes neurodegeneration after traumatic brain injury via inhibition of autophagosome-lysosome fusion (PMID: 38348540). Mutation in DNALI1 Causes Male Infertility with Severe Oligoasthenoteratozoospermia in Humans (PMID: 38212584).

Publikationen

Species	Application	Title
rat	WB,IF	Front Cell Dev Biol; PIH1D3-knockout rats exhibit full ciliopathy features and dysfunctional pre-assembly and loading of dynein arms in motile cilia; Authors - Tingting Zhang
mouse	WB	Development; CCDC183 is essential for cytoplasmic invagination around the flagellum during spermiogenesis and male fertility; Authors - Keisuke Shimada
human	IF	Clin Genet; DNAH2 is a novel candidate gene associated with multiple morphological abnormalities of the sperm flagella.; Authors - Yang Li
mouse	WB,IF	Basic Clin Androl; Dnali1 is required for sperm motility and male fertility in mice; Authors - Yiling Zhou
mouse	WB	Exp Anim; The motor domain of testis-enriched kinesin KIF9 is essential for its localization in the mouse flagellum.; Authors - Haruhiko Miyata
mouse	WB,IF,CoIP	Elife; DNALI1 interacts with the MEIG1/PACRG complex within the manchette and is required for proper sperm flagellum assembly in mice; Authors - Yi Tian Yap ; KO Validated