DNASE1L3 Monoklonaler Antikörper

DNASE1L3 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A4D4

Kat-Nr. : 67041-1-PBS

Synonyme

1A4D4, Deoxyribonuclease gamma, Deoxyribonuclease I-like 3, DHP2, DNAS1L3

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  WB analysis using 67041-1-Ig (same clone as 67041-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67041-1-Ig (DNASE1L3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67041-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67041-1-Ig (same clone as 67041-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67041-1-Ig (DNASE1L3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67041-1-PBS in a different storage buffer formulation.

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  WB analysis of Daudi using 67041-1-Ig (same clone as 67041-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 67041-1-Ig (DNASE1L3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67041-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 67041-1-Ig (same clone as 67041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67041-1-Ig (DNASE1L3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67041-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 67041-1-Ig (same clone as 67041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67041-1-Ig (DNASE1L3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67041-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 67041-1-Ig (same clone as 67041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67041-1-Ig (DNASE1L3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67041-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 67041-1-Ig (same clone as 67041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67041-1-Ig (DNASE1L3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67041-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67041-1-PBS bindet in WB, IHC, ELISA DNASE1L3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DNASE1L3 fusion protein Ag28187
• Vollständiger Name: deoxyribonuclease I-like 3
• Berechnetes Molekulargewicht: 305 aa, 36 kDa
• Beobachtetes Molekulargewicht: 33-38 kDa
• GenBank-Zugangsnummer: BC015831
• Gene symbol: DNASE1L3
• Gene ID (NCBI): 1776
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Deoxyribonuclease 1-like 3 (DNASE1L3, DNase gamma, DNase Y, LS-DNase) is member of a DNASE1 protein family that is identified by similar biochemical properties such as Ca2+/Mg2+- dependency and an optimal pH of about 7.0 as well as by a high similarity in their nucleic acid and amino acid sequences (PMID:157967140). It is an endonuclease and cleaves double-stranded DNA (and single-stranded DNA) producing 3′-OH/5′-P ends, which is Ca2+/Mg2+-dependent and inhibited by Zn2+, but not by monomeric actin (PMID:9665719). In addition, it translocates from the endoplasmic reticulum to the nucleus during apoptosis (PMID: 23229555). DNASE1L3 can be located either in the cytoplasm or in the nucleus.