DNASE2 Polyklonaler Antikörper

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• KD/KO Validated

DNASE2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 15934-1-AP

Synonyme

Acid DNase, Deoxyribonuclease 2 alpha, Deoxyribonuclease II alpha, DNase II alpha, DNASE2, DNASE2A, DNL, DNL2, Lysosomal DNase II, R31240_2

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Galerie der Validierungsdaten

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  WB analysis using 15934-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15934-1-AP (DNASE2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 15934-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15934-1-AP (DNASE2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15934-1-AP

  WB result of DNASE2 antibody (15934-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNASE2 transfected HeLa cells.

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  IP experiment of U-87 MG using 15934-1-AP

  IP result of anti-DNASE2(IP:15934-1-AP, 4ug; Detection:15934-1-AP 1:500) with U-87 MG cells lysate 1160 ug.

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  IP experiment of HeLa using 15934-1-AP

  IP result of anti-DNASE2(IP:15934-1-AP, 4ug; Detection:15934-1-AP 1:500) with HeLa cells lysate 1360 ug.

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  IHC staining of mouse kidney using 15934-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15934-1-AP (DNASE2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 15934-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15934-1-AP (DNASE2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15934-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DNASE2 antibody (15934-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, LNCaP-Zellen, MDA-MB-231-Zellen, U-87 MG-Zellen
• Erfolgreiche IP: U-87 MG-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

15934-1-AP bindet in WB, IP, IHC, IF, ELISA DNASE2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNASE2 fusion protein Ag8781
• Vollständiger Name: deoxyribonuclease II, lysosomal
• Berechnetes Molekulargewicht: 360 aa, 40 kDa
• Beobachtetes Molekulargewicht: 32-40 kDa
• GenBank-Zugangsnummer: BC010419
• Gene symbol: DNASE2
• Gene ID (NCBI): 1777
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNASE2, or 'acid DNase,' hydrolyzes DNA under acidic conditions and is one of 2 distinct mammalian DNases. It hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. It has a key role in the degradation of nuclear DNA in cellular apoptosis during development [PMID:18812394]. Besieds, it is necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells. It has been demonstrated to be responsible forDNA degradation in apoptotic cells engulfed by macrophages and exogenous DNA on the skin surface [PMID:23019102]. DNASE2 exists some isoforms with MV 39 and 34 kDa. (PMID: 14644493)

Publikationen

Species	Application	Title
mouse	WB	J Biol Chem; Autolysosomal degradation of cytosolic chromatin fragments antagonizes oxidative stress-induced senescence.; Authors - Xiaojuan Han
human	WB	Pharmacol Res; Oroxylin A regulates cGAS DNA hypermethylation induced by methionine metabolism to promote HSC senescence; Authors - Danli Zhao
human,mouse	WB	Mol Nutr Food Res; Mangiferin protects DNase 2 abundance via Nrf2 activation to prevent cytosolic mtDNA accumulation during liver injury; Authors - Lisi Li