Dynamin 1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Dynamin 1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 18205-1-AP

Synonyme

DNM, DNM1, dynamin 1, Dynamin1

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 18205-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18205-1-AP (Dynamin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 18205-1-AP

  WB result of Dynamin 1 antibody (18205-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Dynamin 1 transfected A549 cells.

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  WB analysis of NIH/3T3 using 18205-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18205-1-AP (Dynamin 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 18205-1-AP

  IP Result of anti-Dynamin 1 (IP:18205-1-AP, 4ug; Detection:18205-1-AP 1:2000) with mouse brain tissue lysate 4000ug.

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  IHC staining of rat brain using 18205-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18205-1-AP (Dynamin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 18205-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18205-1-AP (Dynamin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18205-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18205-1-AP (Dynamin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18205-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18205-1-AP (Dynamin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Neuro-2a using 18205-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using 18205-1-AP (Dynamin 1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, A549-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Rattenhirngewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Neuro-2a-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below

Produktinformation

18205-1-AP bindet in WB, IP, IHC, IF, ELISA Dynamin 1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Fisch, Huhn
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Dynamin 1 fusion protein Ag12827
• Vollständiger Name: dynamin 1
• Berechnetes Molekulargewicht: 851 aa, 96 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC063850
• Gene symbol: DNM1
• Gene ID (NCBI): 1759
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Elife; Amphiphysin I cleavage by asparagine endopeptidase leads to tau hyperphosphorylation and synaptic dysfunction.; Authors - Xingyu Zhang
fish	WB	Int J Mol Sci; Dietary Nano-ZnO Is Absorbed via Endocytosis and ZIP Pathways, Upregulates Lipogenesis, and Induces Lipotoxicity in the Intestine of Yellow Catfish.; Authors - Shu-Wei Chen
human	WB	Commun Biol; The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells; Authors - Christopher Noyes ; KD Validated
human	WB	Neurobiol Dis; Asparagine endopeptidase cleaves synaptojanin 1 and triggers synaptic dysfunction in Parkinson's disease.; Authors - Li Zou
chicken	WB	J Trace Elem Med Biol; Cu-induced mitochondrial dysfunction is mediated by abnormal mitochondrial fission through oxidative stress in primary chicken embryo hepatocytes.; Authors - Liuyan Wu