DRP1 (N-terminal) Polyklonaler Antikörper

DRP1 (N-terminal) Polyklonal Antikörper für FC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 26187-1-AP

Synonyme

DLP1, DNM1L, DNM1L,DLP1, DRP1, DRP1 (N-terminal), dynamin 1 like, Dynamin 1 like protein, Dynamin like protein

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Galerie der Validierungsdaten

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  WB analysis using 26187-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26187-1-AP (DRP1 (N-terminal) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 26187-1-AP

  IP Result of anti-DNM1L,DLP1 (IP:26187-1-AP, 4ug; Detection:26187-1-AP 1:500) with mouse brain tissue lysate 4000ug.

• 

  FC experiment of HeLa using 26187-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human DRP1 (N-terminal) (26187-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

26187-1-AP bindet in WB, IP, FC, ELISA DRP1 (N-terminal) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DRP1 (N-terminal) fusion protein Ag24249
• Vollständiger Name: dynamin 1-like
• Berechnetes Molekulargewicht: 736 aa, 82 kDa
• Beobachtetes Molekulargewicht: 70-78 kDa
• GenBank-Zugangsnummer: BC024590
• Gene symbol: DNM1L
• Gene ID (NCBI): 10059
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNM1L(Dynamin-1-like protein) , also known as dynamin-related protein 1 (DRP1), belongs to the dynamin family of large GTPases that mediate membrane remodeling during a variety of cellular processes. DNM1L has an important role in the division of growing mitochondria and peroxisomes and also mediates outer mitochondrial membrane fission in mammalian cells. DNM1L is ubiquitously expressed with abundant expression in skeletal muscle, heart, kidney and brain.Variable isoforms of DNM1L have been reported in a tissue-specific manner due to the alternative splicing.

Publikationen

Species	Application	Title
		Mol Cell; An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation; Authors - Stephanie Pei Tung Yiu
human	WB	EMBO Mol Med; High-throughput screening identifies suppressors of mitochondrial fragmentation in OPA1 fibroblasts.; Authors - Emma Cretin
human	WB	Biochem Biophys Res Commun; DUSP1 overexpression attenuates renal tubular mitochondrial dysfunction by restoring Parkin-mediated mitophagy in diabetic nephropathy.; Authors - Chang Lu
human	WB	Asian J Androl; Mannose inhibits the growth of prostate cancer through a mitochondrial mechanism.; Authors - Yu-Lin Deng
	WB	Environ Pollut; Mitochondrial dynamics disruption: Unraveling Dinotefuran's impact on cardiotoxicity; Authors - Jiaping Zhu