• Featured Product
  • KD/KO Validated

DNMT1 Polyklonaler Antikörper

DNMT1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (5)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(96)

Kat-Nr. : 24206-1-AP

Synonyme

AIM, CXXC9, CXXC-type zinc finger protein 9, DNA (cytosine-5)-methyltransferase 1, DNMT



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, C6-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen
Erfolgreiche IPHEK-293-Zellen
Erfolgreiche Detektion in IHChumanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

24206-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA DNMT1 und zeigt Reaktivität mit human, Ratten

Getestete Reaktivität human, Ratte
In Publikationen genannte Reaktivitäthuman, Hausschwein, Huhn, Maus, Ratte, Zebrafisch, Ziege
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen DNMT1 fusion protein Ag19116
Vollständiger Name DNA (cytosine-5-)-methyltransferase 1
Berechnetes Molekulargewicht 1632 aa, 185 kDa
Beobachtetes Molekulargewicht180-200 kDa
GenBank-ZugangsnummerBC126227
Gene symbol DNMT1
Gene ID (NCBI) 1786
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNA methylation is a major epigenetic modification that regulates gene expression. DNMT1, the maintenance DNA methylation enzyme, is the primary enzyme responsible for copying methylation patterns after DNA replication. DNMT1 is required for X chromosome inactivation, imprinting, genomic methylation and proper embryonic development. Overexpression of DNMT1 has been reported in various cancers. DNMT1 exists some isoforms with MW 184 kDa and 145 kDa. (PMID: 10753866)

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for DNMT1 antibody 24206-1-APProtokoll herunterladen
IHC protocol for DNMT1 antibody 24206-1-APProtokoll herunterladenl
IF protocol for DNMT1 antibody 24206-1-APProtokoll herunterladen
IP protocol for DNMT1 antibody 24206-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
rat,humanWB

Environ Int

Manganese induces podocyte injury through regulating MTDH/ALKBH5/NLRP10 axis: Combined analysis at epidemiology and molecular biology levels

Authors - Qiuju Liang
humanWB

Cell Death Dis

Long noncoding RNA LINC01594 inhibits the CELF6-mediated splicing of oncogenic CD44 variants to promote colorectal cancer metastasis

Authors - Bowen Liu
zebrafishWB

Chemosphere

Cyhexatin causes developmental toxic effects by disrupting endocrine system and inducing behavioral inhibition, apoptosis and DNA hypomethylation in zebrafish (Danio rerio) larvae

Authors - Fang Jiao
humanWB,IF,ChIP

Bioact Mater

Matrix stiffness exacerbates the proinflammatory responses of vascular smooth muscle cell through the DDR1-DNMT1 mechanotransduction axis.

Authors - Jin Wang
  • KO Validated
humanCoIP

Clin Transl Med

Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.

Authors - Lijie Zhou
humanWB

J Transl Med

The role of TET2-mediated ROBO4 hypomethylation in the development of diabetic retinopathy

Authors - Liangliang Zhao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Roy (Verified Customer) (09-17-2025)

Great band at the expected size. The dilution used was 1/1000 with an 1h incubation at Room Temperature

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HeLa
DNMT1 Antibody Western Blot validation (1/1000 dilution) in HeLa (Cat no:24206-1-AP)
FH

Paula (Verified Customer) (12-14-2021)

good bands at the right molecular weight, but a lot of smear

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: fetal rat lung tissue
DNMT1 Antibody Western Blot validation (1:500 dilution) in fetal rat lung tissue (Cat no:24206-1-AP)