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DNMT1 Polyklonaler Antikörper
DNMT1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Ratte und mehr (5)
Anwendung
WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 24206-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HeLa-Zellen, C6-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen |
| Erfolgreiche IP | HEK-293-Zellen |
| Erfolgreiche Detektion in IHC | humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF/ICC | HepG2-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunhistochemie (IHC) | IHC : 1:50-1:500 |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 12 publications below |
| WB | See 86 publications below |
| IHC | See 5 publications below |
| IF | See 10 publications below |
| CoIP | See 2 publications below |
| ChIP | See 5 publications below |
| RIP | See 1 publications below |
Produktinformation
24206-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA DNMT1 und zeigt Reaktivität mit human, Ratten
| Getestete Reaktivität | human, Ratte |
| In Publikationen genannte Reaktivität | human, Hausschwein, Huhn, Maus, Ratte, Zebrafisch, Ziege |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | DNMT1 fusion protein Ag19116 |
| Vollständiger Name | DNA (cytosine-5-)-methyltransferase 1 |
| Berechnetes Molekulargewicht | 1632 aa, 185 kDa |
| Beobachtetes Molekulargewicht | 180-200 kDa |
| GenBank-Zugangsnummer | BC126227 |
| Gene symbol | DNMT1 |
| Gene ID (NCBI) | 1786 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
DNA methylation is a major epigenetic modification that regulates gene expression. DNMT1, the maintenance DNA methylation enzyme, is the primary enzyme responsible for copying methylation patterns after DNA replication. DNMT1 is required for X chromosome inactivation, imprinting, genomic methylation and proper embryonic development. Overexpression of DNMT1 has been reported in various cancers. DNMT1 exists some isoforms with MW 184 kDa and 145 kDa. (PMID: 10753866)
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for DNMT1 antibody 24206-1-AP | Protokoll herunterladen |
| IHC protocol for DNMT1 antibody 24206-1-AP | Protokoll herunterladenl |
| IF protocol for DNMT1 antibody 24206-1-AP | Protokoll herunterladen |
| IP protocol for DNMT1 antibody 24206-1-AP | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Environ Int Manganese induces podocyte injury through regulating MTDH/ALKBH5/NLRP10 axis: Combined analysis at epidemiology and molecular biology levels | ||
Cell Death Dis Long noncoding RNA LINC01594 inhibits the CELF6-mediated splicing of oncogenic CD44 variants to promote colorectal cancer metastasis | ||
Chemosphere Cyhexatin causes developmental toxic effects by disrupting endocrine system and inducing behavioral inhibition, apoptosis and DNA hypomethylation in zebrafish (Danio rerio) larvae | ||
Bioact Mater Matrix stiffness exacerbates the proinflammatory responses of vascular smooth muscle cell through the DDR1-DNMT1 mechanotransduction axis.
| ||
Clin Transl Med Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1. | ||
J Transl Med The role of TET2-mediated ROBO4 hypomethylation in the development of diabetic retinopathy |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Roy (Verified Customer) (09-17-2025) | Great band at the expected size. The dilution used was 1/1000 with an 1h incubation at Room Temperature
|
FH Paula (Verified Customer) (12-14-2021) | good bands at the right molecular weight, but a lot of smear
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