DNMT1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DNMT1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24206-1-PBS

Synonyme

AIM, CXXC9, CXXC-type zinc finger protein 9, DNA (cytosine-5)-methyltransferase 1, DNMT

---

• 

  WB analysis using 24206-1-AP (same clone as 24206-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24206-1-AP (DNMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24206-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 24206-1-AP (same clone as 24206-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24206-1-AP (DNMT1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24206-1-PBS in a different storage buffer formulation.

• 

  WB analysis of C6 using 24206-1-AP (same clone as 24206-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 24206-1-AP (DNMT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24206-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 24206-1-AP (same clone as 24206-1-PBS)

  IP result of anti-DNMT1 (IP:24206-1-AP, 4ug; Detection:24206-1-AP 1:500) with HEK-293 cells lysate 1400 ug. This data was developed using the same antibody clone with 24206-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 24206-1-AP (same clone as 24206-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24206-1-AP (DNMT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24206-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 24206-1-AP (same clone as 24206-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24206-1-AP (DNMT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24206-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 24206-1-AP (same clone as 24206-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24206-1-AP (DNMT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24206-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 24206-1-AP (same clone as 24206-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24206-1-AP (DNMT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24206-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 24206-1-AP (same clone as 24206-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DNMT1 antibody (24206-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 24206-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

24206-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA DNMT1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNMT1 fusion protein Ag19116
• Vollständiger Name: DNA (cytosine-5-)-methyltransferase 1
• Berechnetes Molekulargewicht: 1632 aa, 185 kDa
• Beobachtetes Molekulargewicht: 180-200 kDa
• GenBank-Zugangsnummer: BC126227
• Gene symbol: DNMT1
• Gene ID (NCBI): 1786
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNA methylation is a major epigenetic modification that regulates gene expression. DNMT1, the maintenance DNA methylation enzyme, is the primary enzyme responsible for copying methylation patterns after DNA replication. DNMT1 is required for X chromosome inactivation, imprinting, genomic methylation and proper embryonic development. Overexpression of DNMT1 has been reported in various cancers. DNMT1 exists some isoforms with MW 184 kDa and 145 kDa. (PMID: 10753866)