DNMT1 Monoklonaler Antikörper

DNMT1 Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2G5C5

Kat-Nr. : 68485-1-PBS

Synonyme

AIM, CXXC9, DNA methyltransferase HsaI, DNA MTase HsaI, DNMT, DNMT1, FLJ16293, M.HsaI, MCMT

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  WB analysis using 68485-1-Ig (same clone as 68485-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68485-1-Ig (DNMT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68485-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68485-1-PBS bindet in WB, Indirect ELISA DNMT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DNMT1 fusion protein Ag19116
• Vollständiger Name: DNA (cytosine-5-)-methyltransferase 1
• Berechnetes Molekulargewicht: 1632 aa, 185 kDa
• Beobachtetes Molekulargewicht: 180-200 kDa
• GenBank-Zugangsnummer: BC126227
• Gene symbol: DNMT1
• Gene ID (NCBI): 1786
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNA methylation is a major epigenetic modification that regulates gene expression. DNMT1, the maintenance DNA methylation enzyme, is the primary enzyme responsible for copying methylation patterns after DNA replication. DNMT1 is required for X chromosome inactivation, imprinting, genomic methylation and proper embryonic development. Overexpression of DNMT1 has been reported in various cancers. DNMT1 exists some isoforms with MW 184 kDa and 145 kDa. (PMID: 10753866)