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DNMT1 Rekombinanter Antikörper

DNMT1 Rekombinant Antikörper für WB, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240302E2

Kat-Nr. : 82678-2-PBS

Synonyme

240302E2, AIM, CXXC9, CXXC-type zinc finger protein 9, DNA (cytosine-5)-methyltransferase 1

Filter:
  • Western Blot (WB) analysis of various lysates using DNMT1 Recombinant antibody (82678-2-RR)

    WB analysis using 82678-2-RR (same clone as 82678-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82678-2-RR (DNMT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82678-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using DNMT1 Recombinant antibody (82678-2-RR)

    FC experiment of HeLa using 82678-2-RR (same clone as 82678-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug DNMT1 Recombinant antibody (82678-2-RR, Clone:240302E2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82678-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82678-2-RR

    Affinity and Kinetic Characterization of 82678-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82678-2-RR against Human DNMT1 were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

82678-2-PBS bindet in WB, FC (Intra), Indirect ELISA DNMT1 und zeigt Reaktivität mit human, Ratten

Getestete Reaktivität human, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen DNMT1 fusion protein Ag29479
Vollständiger Name DNA (cytosine-5-)-methyltransferase 1
Berechnetes Molekulargewicht 1632 aa, 185 kDa
GenBank-ZugangsnummerBC126227
Gene symbol DNMT1
Gene ID (NCBI) 1786
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.