DNMT2 Polyklonaler Antikörper

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• KD/KO Validated

DNMT2 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19221-1-PBS

Synonyme

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  WB analysis of HeLa using 19221-1-AP (same clone as 19221-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 19221-1-AP (DNMT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19221-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 19221-1-AP (same clone as 19221-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19221-1-AP (DNMT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19221-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 19221-1-AP (same clone as 19221-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19221-1-AP (DNMT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19221-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 19221-1-AP (same clone as 19221-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19221-1-AP (DNMT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19221-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 19221-1-AP (same clone as 19221-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 19221-1-AP (DNMT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19221-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

19221-1-PBS bindet in WB, Indirect ELISA DNMT2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNMT2 fusion protein Ag5571
• Vollständiger Name: tRNA aspartic acid methyltransferase 1
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 43-45 kDa
• GenBank-Zugangsnummer: BC047733
• Gene symbol: DNMT2
• Gene ID (NCBI): 1787
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Dnmt2 was initially assigned a member of the DNA methyltransferase family on the basis of its extensive homology with eukaryotic and prokaryotic DNA-(cytosine C5)-methyltransferases . However, in the apparent absence of a phenotype in dnmt2 knockout cells, Dnmt2's possible biological function remained unknown , even though Dnmt2 is strongly conserved and it is found in species ranging from Schizosaccharomyces pombe to human. Later very weak, residual DNA methylation activity was found with enzymes from different species. The finding that Dnmt2 is an active RNA methyltransferase capable of methylating the C38 position of the tRNA Asp came as a surprise . However, still no cellular function of the tRNA Asp methylation has been found, although in Zebrafish Dnmt2 knock-down caused a developmental phenotype . It is very intriguing that an enzyme that looks like a DNA methyltransferase can methylate RNA, in particular since the RNA and DNA specific m5 C methyltransferases use different catalytic residues and a different mechanism for the methyl transfer reaction . the predicted molecular weight of Dnmt2 is 45 kDa, but it may migrate to the 40 kDa (PMID: 18567810 )