DNMT3A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DNMT3A Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20954-1-PBS

Synonyme

M.HsaIIIA, EC:2.1.1.37, EC:2.1.1.-, DNA MTase HsaIIIA, DNA (cytosine-5)-methyltransferase 3A

---

• 

  WB analysis using 20954-1-AP (same clone as 20954-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20954-1-AP (DNMT3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 20954-1-AP (same clone as 20954-1-PBS)

  WB result of DNMT3A antibody (20954-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNMT3A transfected HepG2 cells. This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 20954-1-AP (same clone as 20954-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20954-1-AP (DNMT3A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 20954-1-AP (same clone as 20954-1-PBS)

  IP result of anti-DNMT3A (IP:20954-1-AP, 4ug; Detection:20954-1-AP 1:5000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 20954-1-AP (same clone as 20954-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 20954-1-AP (same clone as 20954-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 20954-1-AP (same clone as 20954-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 20954-1-AP (same clone as 20954-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 20954-1-AP (same clone as 20954-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 20954-1-AP (same clone as 20954-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 20954-1-AP (same clone as 20954-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 20954-1-AP (same clone as 20954-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

• 

  IF Staining of K-562 using 20954-1-AP (same clone as 20954-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using DNMT3A antibody (20954-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 20954-1-AP (same clone as 20954-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DNMT3A antibody (20954-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

20954-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA DNMT3A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNMT3A fusion protein Ag15111
• Vollständiger Name: DNA (cytosine-5-)-methyltransferase 3 alpha
• Berechnetes Molekulargewicht: 912 aa, 102 kDa
• Beobachtetes Molekulargewicht: 120-130 kDa
• GenBank-Zugangsnummer: BC043617
• Gene symbol: DNMT3A
• Gene ID (NCBI): 1788
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNA methylation in vertebrate animals is an epigenetic modification that is important for embryonic development, imprinting, and the inactivation of X chromosomes. DNA methylation is catalyzed by a family of DNA methyltransferases (DNMTs) that include the maintenance enzyme DNMT1 and de novo methyltransferases DNMT3a and DNMT3b. The overexpression of DNMT1, DNMT3a, and DNMT3b has been reported in various malignancies, including gastric, urothelial, and lung cancers, and may be related to tumorigenesis, tumor progression, and poor survival. Two isoforms of DNMT3a exist: the full-length DNMT3a, and the shorter form DNMT3a2 which lacks the N-terminal fragment. DNMT3a is expressed ubiquitously at low levels, while DNMT3a2 is specially expressed at high levels in embryonic stem cells and shows restricted expression in tissues known to undergo de novo methylation including testis and ovary. This antibody was raised against the N-terminal region of human DNMT3a. It is expected to detect the 120-130 kDa DNMT3a but not 72-100 kDa DNMT3a2.