DNMT3A Rekombinanter Antikörper

DNMT3A Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

242257E1

Kat-Nr. : 81474-5-RR

Synonyme

EC:2.1.1.37, EC:2.1.1.-, DNA MTase HsaIIIA, DNA (cytosine-5)-methyltransferase 3A, Cysteine methyltransferase DNMT3A

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  WB analysis using 81474-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81474-5-RR (DNMT3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 81474-5-RR

  rat testis tissue were subjected to SDS PAGE followed by western blot with 81474-5-RR (DNMT3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 81474-5-RR

  Biolayer interferometry (BLl) kinetic assays of 81474-5-RR against Human DNMT3A were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, Rattenhodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

81474-5-RR bindet in WB, ELISA DNMT3A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DNMT3A fusion protein Ag15111
• Vollständiger Name: DNA (cytosine-5-)-methyltransferase 3 alpha
• Berechnetes Molekulargewicht: 912 aa, 102 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC043617
• Gene symbol: DNMT3A
• Gene ID (NCBI): 1788
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNA methylation in vertebrate animals is an epigenetic modification that is important for embryonic development, imprinting, and the inactivation of X chromosomes. DNA methylation is catalyzed by a family of DNA methyltransferases (DNMTs) that include the maintenance enzyme DNMT1 and de novo methyltransferases DNMT3a and DNMT3b. The overexpression of DNMT1, DNMT3a, and DNMT3b has been reported in various malignancies, including gastric, urothelial, and lung cancers, and may be related to tumorigenesis, tumor progression, and poor survival. Two isoforms of DNMT3a exist: the full-length DNMT3a, and the shorter form DNMT3a2 which lacks the N-terminal fragment. DNMT3a is expressed ubiquitously at low levels, while DNMT3a2 is specially expressed at high levels in embryonic stem cells and shows restricted expression in tissues known to undergo de novo methylation including testis and ovary. This antibody was raised against the N-terminal region of human DNMT3a. It is expected to detect the 120-130 kDa DNMT3a but not 72-100 kDa DNMT3a2.