DNMT3B Polyklonaler Antikörper

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• KD/KO Validated

DNMT3B Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26971-1-PBS

Synonyme

ICF, EC:2.1.1.37, DNMT3, DNMT, DNA (cytosine-5)-methyltransferase 3B

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  WB analysis using 26971-1-AP (same clone as 26971-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26971-1-AP (DNMT3B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26971-1-PBS in a different storage buffer formulation.

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  WB analysis using 26971-1-AP (same clone as 26971-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26971-1-AP (DNMT3B antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26971-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 26971-1-AP (same clone as 26971-1-PBS)

  IP result of anti-DNMT3B (IP:26971-1-AP, 4ug; Detection:26971-1-AP 1:5000) with K-562 cells lysate 1800 ug. This data was developed using the same antibody clone with 26971-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 26971-1-AP (same clone as 26971-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26971-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 26971-1-AP (same clone as 26971-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26971-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 26971-1-AP (same clone as 26971-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26971-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 26971-1-AP (same clone as 26971-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26971-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 26971-1-AP (same clone as 26971-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26971-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 26971-1-AP (same clone as 26971-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26971-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

26971-1-PBS bindet in WB, IHC, IP, Indirect ELISA DNMT3B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNMT3B fusion protein Ag25117
• Vollständiger Name: DNA (cytosine-5-)-methyltransferase 3 beta
• Berechnetes Molekulargewicht: 95 kDa
• Beobachtetes Molekulargewicht: 85-96 kDa
• GenBank-Zugangsnummer: NM_001207055
• Gene symbol: DNMT3B
• Gene ID (NCBI): 1789
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNMT3B is a DNA-(cytosine C5)-methyltransferase that is responsible for establishing the methylation patterns in cooperation with DNMT3A through the de novo methylation pathway. It is essential for the establishment of DNA methylation patterns during development. DNMT3B contains a C-terminal catalytic domain and an N-terminal regulatory domain, which are involved in targeting chromatin to regulate its function (PMID: 31547729). DNMT3B has 8 isoforms with the molecular mass of 80-96 kDa.