DOCK11 Rekombinanter Antikörper

DOCK11 Rekombinant Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242575H5

Kat-Nr. : 85022-3-PBS

Synonyme

242575H5, ACG, bB128O4.1, dedicator of cytokinesis 11, ZIZ2

---

• 

  WB analysis using 85022-3-RR (same clone as 85022-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85022-3-RR (DOCK11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85022-3-PBS in a different storage buffer formulation.

• 

  WB analysis using 85022-3-RR (same clone as 85022-3-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 85022-3-RR (DOCK11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85022-3-PBS in a different storage buffer formulation.

• 

  WB analysis using 85022-3-RR (same clone as 85022-3-PBS)

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 85022-3-RR (DOCK11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85022-3-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 85022-3-RR (same clone as 85022-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85022-3-RR (DOCK11 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85022-3-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 85022-3-RR (same clone as 85022-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85022-3-RR (DOCK11 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85022-3-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01759-2

  Cytometric bead array standard curve of MP01759-2, DOCK11 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85022-3-PBS. Detection antibody: 85022-1-PBS. Standard: Ag35831. Range: 0.781-100 ng/mL.

• 

  Affinity and Kinetic Characterization of 85022-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85022-3-RR against Human DOCK11 were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

Produktinformation

85022-3-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA DOCK11 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DOCK11 fusion protein Ag35831
• Vollständiger Name: dedicator of cytokinesis 11
• Berechnetes Molekulargewicht: 238 kDa
• Beobachtetes Molekulargewicht: 238 kDa
• GenBank-Zugangsnummer: NM_144658
• Gene symbol: DOCK11
• Gene ID (NCBI): 139818
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DOCK11(also known as Zizimin2) is a member of CDM (Ced-5, DOCK180, and Myoblast City) family guanine nucleotide exchange factor mainly expressed in immune cells. As a guanine nucleotide exchange factor, DOCK11 activated the rho family GTPase cell division cycle 42 (CDC42), resulting in cytoskeletal reorganization.