DOCK11 Rekombinanter Antikörper

DOCK11 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242575H5

Kat-Nr. : 85022-3-RR

Synonyme

242575H5, ACG, bB128O4.1, dedicator of cytokinesis 11, ZIZ2

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  WB analysis using 85022-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85022-3-RR (DOCK11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85022-3-RR

  K-562 cells were subjected to SDS PAGE followed by western blot with 85022-3-RR (DOCK11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85022-3-RR

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 85022-3-RR (DOCK11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 85022-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85022-3-RR (DOCK11 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 85022-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85022-3-RR (DOCK11 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 85022-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85022-3-RR against Human DOCK11 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, K-562-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85022-3-RR bindet in WB, IHC, ELISA DOCK11 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DOCK11 fusion protein Ag35831
• Vollständiger Name: dedicator of cytokinesis 11
• Berechnetes Molekulargewicht: 238 kDa
• Beobachtetes Molekulargewicht: 238 kDa
• GenBank-Zugangsnummer: NM_144658
• Gene symbol: DOCK11
• Gene ID (NCBI): 139818
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DOCK11(also known as Zizimin2) is a member of CDM (Ced-5, DOCK180, and Myoblast City) family guanine nucleotide exchange factor mainly expressed in immune cells. As a guanine nucleotide exchange factor, DOCK11 activated the rho family GTPase cell division cycle 42 (CDC42), resulting in cytoskeletal reorganization.