DOCK2 Monoklonaler Antikörper

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• KD/KO Validated

DOCK2 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D7A8

Kat-Nr. : 66969-1-PBS

Synonyme

dedicator of cytokinesis 2, DOCK2, FLJ46592, KIAA0209

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  WB analysis of Jurkat using 66969-1-Ig (same clone as 66969-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66969-1-Ig (DOCK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66969-1-PBS in a different storage buffer formulation.

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  WB analysis of Ramos using 66969-1-Ig (same clone as 66969-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 66969-1-Ig (DOCK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66969-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66969-1-Ig (same clone as 66969-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66969-1-Ig (DOCK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66969-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66969-1-Ig (same clone as 66969-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66969-1-Ig (DOCK2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66969-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66969-1-Ig (same clone as 66969-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66969-1-Ig (DOCK2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66969-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66969-1-Ig (same clone as 66969-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DOCK2 antibody (66969-1-Ig, Clone: 1D7A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66969-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66969-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA DOCK2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DOCK2 fusion protein Ag28582
• Vollständiger Name: dedicator of cytokinesis 2
• Berechnetes Molekulargewicht: 212 kDa, 38 kDa
• Beobachtetes Molekulargewicht: 200-210 kDa
• GenBank-Zugangsnummer: BC016996
• Gene symbol: DOCK2
• Gene ID (NCBI): 1794
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DOCK2, also named as KIAA0209, belongs to the DOCK family. It is involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. DOCK2 activates RAC1 and RAC2 small GTPases, probably by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. DOCK2 may also participate in IL2 transcriptional activation via the activation of RAC2. The antibody is specific to DOCK2.