DOCK3; MOCA Polyklonaler Antikörper

DOCK3; MOCA Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20683-1-PBS

Synonyme

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  WB analysis using 20683-1-AP (same clone as 20683-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20683-1-AP (DOCK3; MOCA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20683-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 20683-1-AP (same clone as 20683-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 20683-1-AP (DOCK3; MOCA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20683-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 20683-1-AP (same clone as 20683-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 20683-1-AP (DOCK3; MOCA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20683-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 20683-1-AP (same clone as 20683-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20683-1-AP (DOCK3; MOCA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20683-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 20683-1-AP (same clone as 20683-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20683-1-AP (DOCK3; MOCA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20683-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 20683-1-AP (same clone as 20683-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20683-1-AP (DOCK3; MOCA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20683-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20683-1-PBS bindet in WB, IHC, Indirect ELISA DOCK3; MOCA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: dedicator of cytokinesis 3
• Berechnetes Molekulargewicht: 233 kDa
• Beobachtetes Molekulargewicht: 233 kDa
• GenBank-Zugangsnummer: NM_004947
• Gene symbol: DOCK3
• Gene ID (NCBI): 1795
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Dedicator of cytokinesis 3 (DOCK3), also named as MOCA and PBP , is a ~180 kDa protein involved in signaling trasduction. It is a potential guanine nucleotide exchange factor (GEF) which activate some small GTPases by exchanging bound GDP for free GTP. DOCK3 is associated in Alzheimer disease tangles and regulates the accumulation of amyloid precursor protein and beta-amyloid. Overexpression of Dock3 in neural cells promotes axonal outgrowth downstream of brain-derived neurotrophic factor (BDNF) signaling. DOCK3 binds to and inactivates glycogen synthase kinase-3β (GSK-3β) at the plasma membrane, thereby promoteing axon branching and microtubule assembly. By stimulating actin polymerization and microtubule assembly, DOCK3 plays important roles downstream of BDNF signaling in the CNS.