DOCK4 Polyklonaler Antikörper

DOCK4 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 21861-1-AP

Synonyme

dedicator of cytokinesis 4, DOCK4, KIAA0716

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 21861-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21861-1-AP (DOCK4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 21861-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21861-1-AP (DOCK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 21861-1-AP

  IP Result of anti-DOCK4 (IP:21861-1-AP, 5ug; Detection:21861-1-AP 1:500) with HeLa cells lysate 1800ug.

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  IP experiment of HEK-293T using 21861-1-AP

  IP Result of anti-DOCK4 (IP:21861-1-AP, 4ug; Detection:21861-1-AP 1:300) with HEK-293T cells lysate 4000ug.

• 

  IHC staining of human ovary tumor using 21861-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21861-1-AP (DOCK4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 21861-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21861-1-AP (DOCK4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21861-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21861-1-AP (DOCK4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21861-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21861-1-AP (DOCK4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 21861-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21861-1-AP (DOCK4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 21861-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21861-1-AP (DOCK4 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 21861-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21861-1-AP (DOCK4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 21861-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21861-1-AP (DOCK4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 21861-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 21861-1-AP (DOCK4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HEK-293T-Zellen
• Erfolgreiche IP: HeLa-Zellen, HEK-293T-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Prostatakarzinomgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:150-1:600
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

21861-1-AP bindet in WB, IP, IHC, IF DOCK4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DOCK4 fusion protein Ag16516
• Vollständiger Name: dedicator of cytokinesis 4
• Berechnetes Molekulargewicht: 2011 aa, 230 kDa
• Beobachtetes Molekulargewicht: 225 kDa
• GenBank-Zugangsnummer: BC117689
• Gene symbol: DOCK4
• Gene ID (NCBI): 9732
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DOCK4, originally identified as a product of a gene which is deleted during tumor progression, is a member of DOCK180 family proteins. Dock4 has been found recently to be associated with several neuropsychiatric diseases, including autism, dyslexia, and schizophrenia. Multiple studies in fibroblasts then confirmed that Dock4 is capable of controlling cell migration by transducing several upstream signals, such as Wnt, platelet-derived growth factor, and RhoG, toward activation of Rac1. Mutations in this gene have been associated with ovarian, prostate, glioma, and colorectal cancers.

Publikationen

Species	Application	Title
human	WB,IF	Proc Natl Acad Sci U S A; Up-regulated cytotrophoblast DOCK4 contributes to over-invasion in placenta accreta spectrum.; Authors - Leah McNally
human	WB, IHC	Front Cell Dev Biol; USP36-Mediated Deubiquitination of DOCK4 Contributes to the Diabetic Renal Tubular Epithelial Cell Injury via Wnt/β-Catenin Signaling Pathway.; Authors - Suwei Zhu
human	WB	Front Oncol; miR-33b-3p Acts as a Tumor Suppressor by Targeting DOCK4 in Prostate Cancer.; Authors - Yu Mei

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