DOCK5 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DOCK5 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29938-1-PBS

Synonyme

dedicator of cytokinesis 5

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  WB analysis using 29938-1-AP (same clone as 29938-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29938-1-AP (DOCK5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29938-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 29938-1-AP (same clone as 29938-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29938-1-AP (DOCK5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29938-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 29938-1-AP (same clone as 29938-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29938-1-AP (DOCK5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29938-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 29938-1-AP (same clone as 29938-1-PBS)

  WB result of DOCK5 antibody (29938-1-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-DOCK5 transfected A549 cells. This data was developed using the same antibody clone with 29938-1-PBS in a different storage buffer formulation.

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  IP experiment of DU 145 using 29938-1-AP (same clone as 29938-1-PBS)

  IP result of anti-DOCK5 (IP:29938-1-AP, 4ug; Detection:29938-1-AP 1:2000) with DU 145 cells lysate 1280 ug. This data was developed using the same antibody clone with 29938-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 29938-1-AP (same clone as 29938-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29938-1-AP (DOCK5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29938-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 29938-1-AP (same clone as 29938-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 29938-1-AP (DOCK5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29938-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 29938-1-AP (same clone as 29938-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 29938-1-AP (DOCK5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29938-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 29938-1-AP (same clone as 29938-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using DOCK5 antibody (29938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29938-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29938-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA DOCK5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DOCK5 fusion protein Ag32193
• Vollständiger Name: dedicator of cytokinesis 5
• Berechnetes Molekulargewicht: 1870 aa, 215 kDa
• Beobachtetes Molekulargewicht: 190 kDa
• GenBank-Zugangsnummer: BC137175
• Gene symbol: DOCK5
• Gene ID (NCBI): 80005
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DOCK5 is a member of the dedicator of cytokinesis protein family. Members of this family act as guanine nucleotide exchange factors for small Rho family G proteins. DOCK5 is thought to associate with adaptors CRK and CRKL, and function in regulation of intestinal epithelial cell spreading and migration on collagen IV.