DOCK8 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DOCK8 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 11622-1-AP

Synonyme

dedicator of cytokinesis 8, DOCK8, MRD2, ZIR8

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Galerie der Validierungsdaten

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  WB analysis using 11622-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11622-1-AP (DOCK8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 11622-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11622-1-AP (DOCK8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 11622-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11622-1-AP (DOCK8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 11622-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11622-1-AP (DOCK8 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 11622-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11622-1-AP (DOCK8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 11622-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11622-1-AP (DOCK8 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse lung using 11622-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11622-1-AP (DOCK8 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse lung using 11622-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11622-1-AP (DOCK8 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse kidney using 11622-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11622-1-AP (DOCK8 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse kidney using 11622-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11622-1-AP (DOCK8 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, Rattenlungengewebe, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Herzgewebe, Mauslungengewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

11622-1-AP bindet in WB, IP, IHC, IF, ELISA DOCK8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DOCK8 fusion protein Ag2040
• Vollständiger Name: dedicator of cytokinesis 8
• Berechnetes Molekulargewicht: 2031 aa, 231 kDa
• Beobachtetes Molekulargewicht: 230-239 kDa
• GenBank-Zugangsnummer: BC019102
• Gene symbol: DOCK8
• Gene ID (NCBI): 81704
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Background

Dedicator of Cytokinesis 8 (DOCK8) is a protein that regulates the actin cytoskeleton, with particular importance in immune cells and a key role in innate and adaptive immune responses.

 What is the molecular weight of DOCK8?

231 kDa. DOCK8 is a protein composed of 2031 amino acids and is a guanine nucleotide exchange factor (GEF).

 What is the function of DOCK8?

DOCK8 is a member of the DOCK family of proteins, which have a unique DRH2 domain enabling them to act as GEFs and so controlling a range of cellular processes in various signaling pathways (PMID: 12432077). The specific target of DOCK8 is Cell division control protein 42 homolog (Cdc42), a small GTPase that is involved in regulation of the cell cycle and forms a complex. DOCK8 also acts as a scaffold molecule in this complex that initiates actin polymerization via the Wiskott-Aldrich Syndrome protein (WASp) (PubMed: 28028151, PubMed: 22461490).

 What diseases are associated with DOCK8?

The role of DOCK8 in immunity was first identified with the study of DOCK8-deficient patients who presented with combined immunodeficiency (PMID: 19776401; PMID: 20004785). The subsequent study of DOCK8 in immune cells such as T cells, natural killer (NK) cells, and B cells has revealed how it regulates their normal function. This includes the regulation of immune synapse formation, immune cell trafficking, regulation of dendritic cell polarization, and cytokine production (PMID: 28366940).

DOCK8 deficiency is caused by a number of different mutations in the gene. It leads to the autosomal recessive form of the immunodeficiency disease Hyper-IgE syndrome, or Job's syndrome. The symptoms of DOCK8 deficiency include eczema, high levels of serum IgE, hypereosinophilia, and recurrent respiratory and skin infections as a result of impaired immune cell function. 

Publikationen

Species	Application	Title
mouse	WB	Nat Immunol; Heme drives hemolysis-induced susceptibility to infection via disruption of phagocyte functions.; Authors - Rui Martins ; KO Validated
mouse	IF	Cell Death Differ; CCL2 regulation of MST1-mTOR-STAT1 signaling axis controls BCR signaling and B-cell differentiation.; Authors - Lu Yang
mouse	WB	Diabetes; Dedicator of Cytokinesis 5 Regulates Keratinocyte Function and Promotes Diabetic Wound Healing.; Authors - Hua Qu
human	WB, IF, IP	Oncotarget; CD147 promotes Src-dependent activation of Rac1 signaling through STAT3/DOCK8 during the motility of hepatocellular carcinoma cells.; Authors - Shi-Jie Wang ; KD Validated
human	WB	iScience; DOCK8-expressing T follicular helper cells newly generated beyond self-organized criticality cause systemic lupus erythematosus.; Authors - Shunichi Shiozawa ; KO Validated
mouse	WB	MedComm (2020); Ultra-high static magnetic fields cause immunosuppression through disrupting B-cell peripheral differentiation and negatively regulating BCR signaling; Authors - Heng Gu