DOCK8 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DOCK8 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11622-1-PBS

Synonyme

dedicator of cytokinesis 8, MRD2

---

• 

  WB analysis using 11622-1-AP (same clone as 11622-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11622-1-AP (DOCK8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11622-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 11622-1-AP (same clone as 11622-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11622-1-AP (DOCK8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11622-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat lung using 11622-1-AP (same clone as 11622-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 11622-1-AP (DOCK8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11622-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 11622-1-AP (same clone as 11622-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11622-1-AP (DOCK8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11622-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 11622-1-AP (same clone as 11622-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11622-1-AP (DOCK8 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11622-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 11622-1-AP (same clone as 11622-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11622-1-AP (DOCK8 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11622-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 11622-1-AP (same clone as 11622-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11622-1-AP (DOCK8 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11622-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 11622-1-AP (same clone as 11622-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11622-1-AP (DOCK8 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11622-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 11622-1-AP (same clone as 11622-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 11622-1-AP (DOCK8 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11622-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 11622-1-AP (same clone as 11622-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 11622-1-AP (DOCK8 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11622-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

11622-1-PBS bindet in WB, IHC, Indirect ELISA DOCK8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DOCK8 fusion protein Ag2040
• Vollständiger Name: dedicator of cytokinesis 8
• Berechnetes Molekulargewicht: 2031 aa, 231 kDa
• Beobachtetes Molekulargewicht: 230-239 kDa
• GenBank-Zugangsnummer: BC019102
• Gene symbol: DOCK8
• Gene ID (NCBI): 81704
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Background

Dedicator of Cytokinesis 8 (DOCK8) is a protein that regulates the actin cytoskeleton, with particular importance in immune cells and a key role in innate and adaptive immune responses.

 What is the molecular weight of DOCK8?

231 kDa. DOCK8 is a protein composed of 2031 amino acids and is a guanine nucleotide exchange factor (GEF).

 What is the function of DOCK8?

DOCK8 is a member of the DOCK family of proteins, which have a unique DRH2 domain enabling them to act as GEFs and so controlling a range of cellular processes in various signaling pathways (PMID: 12432077). The specific target of DOCK8 is Cell division control protein 42 homolog (Cdc42), a small GTPase that is involved in regulation of the cell cycle and forms a complex. DOCK8 also acts as a scaffold molecule in this complex that initiates actin polymerization via the Wiskott-Aldrich Syndrome protein (WASp) (PubMed: 28028151, PubMed: 22461490).

 What diseases are associated with DOCK8?

The role of DOCK8 in immunity was first identified with the study of DOCK8-deficient patients who presented with combined immunodeficiency (PMID: 19776401; PMID: 20004785). The subsequent study of DOCK8 in immune cells such as T cells, natural killer (NK) cells, and B cells has revealed how it regulates their normal function. This includes the regulation of immune synapse formation, immune cell trafficking, regulation of dendritic cell polarization, and cytokine production (PMID: 28366940).

DOCK8 deficiency is caused by a number of different mutations in the gene. It leads to the autosomal recessive form of the immunodeficiency disease Hyper-IgE syndrome, or Job's syndrome. The symptoms of DOCK8 deficiency include eczema, high levels of serum IgE, hypereosinophilia, and recurrent respiratory and skin infections as a result of impaired immune cell function.