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DOCK8 Rekombinanter Antikörper

DOCK8 Rekombinant Antikörper für IF/ICC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230441A2

Kat-Nr. : CL488-83270-2

Synonyme

230441A2, dedicator of cytokinesis 8, MRD2

Filter:
  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using CoraLite® Plus 488-conjugated DOCK8 Recombinant an (CL488-83270-2)

    IF Staining of THP-1 using CL488-83270-2

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CoraLite® Plus 488 DOCK8 antibody (CL488-83270-2, Clone: 230441A2 ) at dilution of 1:200.

Geprüfte Anwendungen

Erfolgreiche Detektion in IF/ICCTHP-1-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-83270-2 bindet in IF/ICC DOCK8 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen DOCK8 fusion protein Ag34585
Vollständiger Name dedicator of cytokinesis 8
Berechnetes Molekulargewicht 2031 aa, 231 kDa
Beobachtetes Molekulargewicht239 kDa
GenBank-ZugangsnummerBC019102
Gene symbol DOCK8
Gene ID (NCBI) 81704
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DOCK8 is a member of the DOCK family of proteins, which have a unique DRH2 domain enabling them to act as GEFs and so controlling a range of cellular processes in various signaling pathways (PMID: 12432077). The specific target of DOCK8 is Cell division control protein 42 homolog (Cdc42), a small GTPase that is involved in regulation of the cell cycle and forms a complex. DOCK8 also acts as a scaffold molecule in this complex that initiates actin polymerization via the Wiskott-Aldrich Syndrome protein (WASp) (PubMed: 28028151, PubMed: 22461490).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IF protocol for CL Plus 488 DOCK8 antibody CL488-83270-2Protokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen