DOCK9 Polyklonaler Antikörper

DOCK9 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 18987-1-AP

Synonyme

Zizimin-1, ZIZ1, dedicator of cytokinesis 9, Cdc42 guanine nucleotide exchange factor zizimin-1

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  WB analysis of A549 using 18987-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 18987-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 18987-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 18987-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 18987-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 18987-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 18987-1-AP

  IP result of anti-DOCK9 (IP:18987-1-AP, 4ug; Detection:18987-1-AP 1:800) with mouse brain tissue lysate 4000ug.

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  IHC staining of human kidney using 18987-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18987-1-AP (DOCK9 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 18987-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18987-1-AP (DOCK9 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 18987-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18987-1-AP (DOCK9 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 18987-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18987-1-AP (DOCK9 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 18987-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DOCK9 antibody (18987-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, humanes Hirngewebe, humanes Herzgewebe, humanes Plazenta-Gewebe, MCF-7-Zellen, Maushirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

18987-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA DOCK9 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: dedicator of cytokinesis 9
• Berechnetes Molekulargewicht: 239 kDa
• Beobachtetes Molekulargewicht: 200-236 kDa
• GenBank-Zugangsnummer: NM_015296
• Gene symbol: DOCK9
• Gene ID (NCBI): 23348
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DOCK9 is a guanine nucleotide exchange factor (GEF) that activates CDC42 by exchanging bound GDP for free GTP. Its overexpression induces filopodia formation.

Publikationen

Species	Application	Title
human	WB	Commun Biol; Brain endothelial cells promote breast cancer cell extravasation to the brain via EGFR-DOCK4-RAC1 signalling; Authors - Chiara Galloni
human	WB,IHC,IF	Clin Exp Med; DOCK9 as a predictive biomarker linked to angiogenesis and immune response in esophageal squamous cell carcinoma; Authors - Yaqiang Pan ; KD Validated