DOM3Z Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DOM3Z Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 11015-2-AP

Synonyme

DXO, Dom-3 homolog Z, DOM 3Z, DeNADding enzyme DXO, Decapping and exoribonuclease protein

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  WB analysis of mouse testis using 11015-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11015-2-AP (DOM3Z antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 11015-2-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11015-2-AP (DOM3Z antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 11015-2-AP

  IP result of anti-DOM3Z (IP:11015-2-AP, 4ug; Detection:11015-2-AP 1:300) with mouse testis tissue lysate 4000ug.

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  IHC staining of human breast cancer using 11015-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11015-2-AP (DOM3Z antibody) at dilution of 1:50 (under 25x lens).

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  IHC staining of human breast cancer using 11015-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11015-2-AP (DOM3Z antibody) at dilution of 1:50 (under 25x lens).

• 

  IF Staining of HeLa using 11015-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DOM3Z antibody (11015-2-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IF: See 2 publications below

Produktinformation

11015-2-AP bindet in WB, IHC, IF/ICC, IP, ELISA DOM3Z und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DOM3Z fusion protein Ag1491
• Vollständiger Name: dom-3 homolog Z (C. elegans)
• Berechnetes Molekulargewicht: 43.6 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC009344
• Gene symbol: DOM3Z
• Gene ID (NCBI): 1797
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DOM3Z also known as DXO, NG6, is a 396 amino acid ubiquitously expressed protein. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6 and possesses RNA 5'-pyrophosphohydrolase activity. Chromosome 6 contains 170 million base pairs and represents nearly 6% of the human genome. Partial deletion of the q arm of chromosome 6 is associated with early-onset colorectal cancer. DOM3Z is ubiquitously expressed, with high expression in the testis (PMID: 9799600).

Publikationen

Species	Application	Title
human	WB	Cell; 5' End Nicotinamide Adenine Dinucleotide Cap in Human Cells Promotes RNA Decay through DXO-Mediated deNADding.; Authors - Xinfu Jiao ; KO Validated
human	WB	Mol Cell; A mammalian pre-mRNA 5' end capping quality control mechanism and an unexpected link of capping to pre-mRNA processing.; Authors - Xinfu Jiao ; KD Validated
human	WB,IF	RNA; Unraveling regulation and new components of human P-bodies through a protein interaction framework and experimental validation.; Authors - Zheng Dinghai D
human	IF	PLoS One; 2'-O-methylation of the mRNA cap protects RNAs from decapping and degradation by DXO.; Authors - Frédéric Picard-Jean
human	WB	Nat Chem Biol; Structural and mechanistic basis of mammalian Nudt12 RNA deNADding.; Authors - Ewa Grudzien-Nogalska ; KO Validated