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DOT1L Polyklonaler Antikörper

DOT1L Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28145-1-PBS

Synonyme

Lysine N methyltransferase 4, KMT4, KIAA1814, H3 K79 HMTase, DOT1 like protein

Filter:
  • Western Blot (WB) analysis of various lysates using DOT1L Polyclonal antibody (28145-1-AP)

    WB analysis using 28145-1-AP (same clone as 28145-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28145-1-AP (DOT1L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28145-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

28145-1-PBS bindet in WB, Indirect ELISA DOT1L und zeigt Reaktivität mit human, Ratten

Getestete Reaktivität human, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen DOT1L fusion protein Ag27229
Vollständiger Name DOT1-like, histone H3 methyltransferase (S. cerevisiae)
Berechnetes Molekulargewicht 185 kDa
Beobachtetes Molekulargewicht185~200 kDa
GenBank-ZugangsnummerNM_032482
Gene symbol DOT1L
Gene ID (NCBI) 84444
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DOT1L (Disruptor of telomeric silencing 1-like) is the only known histone methyltransferse catalyzing H3K79 methylation which is an activating mark for gene transcription by promoting transcriptional elongation (PMID: 34187895). DOT1L forms a complex with AF10/AF17 and ENL/AF9, is dysregulated in mixed-lineage leukemia (MLLr), and is believed to regulate transcriptional elongation without direct evidence. DOT1L has been reported to directly interact with RNAPII phosphorylated on Ser2 and/or Ser5 of the C-terminal domain (CTD) of its largest subunit. Loss of Dot1L results in yolk sac angiogenesis defects and embryonic lethality (PMID: 18787701).