DPF2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DPF2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 12111-1-AP

Synonyme

BAF45D, BRG1 associated factor 45D, DPF2, Protein requiem, REQ, ubi d4, UBID4, Zinc finger protein ubi d4

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Galerie der Validierungsdaten

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  WB analysis using 12111-1-AP

  Various cells were subjected to SDS PAGE followed by western blot with 12111-1-AP (DPF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 12111-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12111-1-AP (DPF2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12111-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12111-1-AP (DPF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 12111-1-AP

  IP Result of anti-DPF2 (IP:12111-1-AP, 4ug; Detection:12111-1-AP 1:500) with Jurkat cells lysate 1200ug.

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  IHC staining of human lung cancer using 12111-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12111-1-AP (DPF2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pituitary adenoma using 12111-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 12111-1-AP (DPF2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pituitary adenoma using 12111-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 12111-1-AP (DPF2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12111-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12111-1-AP (DPF2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 12111-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12111-1-AP (DPF2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 12111-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12111-1-AP (DPF2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 12111-1-AP

  Immunofluorescent analysis of HepG2 cells, using DPF2 antibody 12111-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 12111-1-AP

  Immunofluorescent analysis of Hela cells, using DPF2 antibody 12111-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen, Maus-Thymusgewebe, Y79-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Hodengewebe, humanes Hypophysenadenom-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Produktinformation

12111-1-AP bindet in WB, IP, IHC, IF, ELISA DPF2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DPF2 fusion protein Ag2756
• Vollständiger Name: D4, zinc and double PHD fingers family 2
• Berechnetes Molekulargewicht: 391 aa, 44 kDa
• Beobachtetes Molekulargewicht: 44-50 kDa
• GenBank-Zugangsnummer: BC014889
• Gene symbol: DPF2
• Gene ID (NCBI): 5977
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DPF2, also named as BAF45D or UBID4, is a 391 amino acid protein, which belongs to the requiem/DPF family. DPF2 may be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells and might also have a role in the development and maturation of lymphoid cells. DPF2 was recently shown to inhibit the myeloid differentiation of hematopoietic stem/progenitor and acute myelogenous leukemia cells.

Publikationen

Species	Application	Title
mouse	IF	Nat Commun; SS18 regulates pluripotent-somatic transition through phase separation.; Authors - Junqi Kuang
human	WB	Anal Chem; Deciphering the Interactome of Histone Marks in Living Cells via Genetic Code Expansion Combined with Proximity Labeling.; Authors - Yepei Huang
human	WB,IHC,IF	Front Neurosci; BAF45D Downregulation in Spinal Cord Ependymal Cells Following Spinal Cord Injury in Adult Rats and Its Potential Role in the Development of Neuronal Lesions.; Authors - Zhenzhen Wang ; KD Validated
human,mouse	WB,IHC,IF	Front Neuroanat; The BAF45D Protein Is Preferentially Expressed in Adult Neurogenic Zones and in Neurons and May Be Required for Retinoid Acid Induced PAX6 Expression.; Authors - Chao Liu ; KD Validated
human	WB	Biosci Biotechnol Biochem; BAF45D knockdown decreases cell viability, inhibits colony formation, induces cell apoptosis and S-phase arrest in human pancreatic cancer cells.; Authors - Zengyi Liu ; KD Validated
human	WB, IF	Biochim Biophys Acta; DPF2 regulates OCT4 protein level and nuclear distribution.; Authors - Chao Liu