DPH2 Polyklonaler Antikörper

DPH2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12367-1-PBS

Synonyme

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  WB analysis of human brain using 12367-1-AP (same clone as 12367-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12367-1-AP (DPH2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12367-1-PBS in a different storage buffer formulation.

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  WB analysis using 12367-1-AP (same clone as 12367-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12367-1-AP (DPH2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12367-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 12367-1-AP (same clone as 12367-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12367-1-AP (DPH2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12367-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse skeletal muscle using 12367-1-AP (same clone as 12367-1-PBS)

  IP result of anti-DPH2 (IP:12367-1-AP, 3ug; Detection:12367-1-AP 1:1000) with mouse skeletal muscle tissue lysate 6000ug. This data was developed using the same antibody clone with 12367-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 12367-1-AP (same clone as 12367-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12367-1-AP (DPH2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12367-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 12367-1-AP (same clone as 12367-1-PBS)

  Immunofluorescent analysis of PC-3 cells using 12367-1-AP (DPH2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12367-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12367-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA DPH2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DPH2 fusion protein Ag3042
• Vollständiger Name: DPH2 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 489 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC016956
• Gene symbol: DPH2
• Gene ID (NCBI): 1802
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2). When overexpressed, suppresses colony formation ability and growth rate of ovarian cancer cells. Mutants of the DPH1, DPH2, and DPH5 (611075) genes, involved in diphthamide biosynthesis, were identified as resistant to both anthrax toxin and diphtheria toxin. This antibody works well in WB, IP, IHC and IF application.