DPP10 Rekombinanter Antikörper

DPP10 Rekombinant Antikörper für WB, IP, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IP, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241446A5

Kat-Nr. : 84127-2-PBS

Synonyme

241446A5, dipeptidyl peptidase 10, Dipeptidyl peptidase IV-related protein 3, Dipeptidyl peptidase X, Dipeptidyl peptidase-like protein 2

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  WB analysis using 84127-2-RR (same clone as 84127-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84127-2-RR (DPP10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84127-2-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA sample test of MP01035-3

  HT-29 cell lysate was measured. The human DPP10 concentration of detected samples was determined to be 3,448.25 ng/mL (based on a 4.4 mg/mL extract load).

• 

  Sandwich ELISA standard curve of MP01035-3

  Sandwich ELISA standard curve of MP01035-3, Human DPP10 Recombinant Matched Antibody Pair - PBS only. 84127-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3231. 84127-3-PBS was HRP conjugated as the detection antibody. Range: 6.25-400 ng/mL

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  IP experiment of pig brain using 84127-2-RR (same clone as 84127-2-PBS)

  IP result of anti-DPP10 (IP:84127-2-RR, 4ug; Detection:84127-2-RR 1:1000) with pig brain tissue lysate 1240 ug. This data was developed using the same antibody clone with 84127-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84127-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 84127-2-PBS against Human DPP10 was performed. The affinity constant is 3.15 nM.

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  Affinity and Kinetic Characterization of 84127-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84127-2-RR against Human DPP10 were performed. The affinity constant is 1.32 nM.

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Geprüfte Anwendungen

Produktinformation

84127-2-PBS bindet in WB, IP, Sandwich ELISA, Indirect ELISA DPP10 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DPP10 fusion protein Ag3231
• Vollständiger Name: dipeptidyl-peptidase 10
• Berechnetes Molekulargewicht: 796 aa, 91 kDa
• Beobachtetes Molekulargewicht: 91 kDa
• GenBank-Zugangsnummer: BC030832
• Gene symbol: DPP10
• Gene ID (NCBI): 57628
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DPP10 is a single-pass type II membrane protein that is a member of the S9B family in clan SC of the serine proteases. DPP10 is an inactive peptidase that modulates the electrophysiological properties, cell-surface expression and subcellular localization of voltage-gated potassium channels.