DPP3 Rekombinanter Antikörper

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• KD/KO Validated

DPP3 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

241360D4

Kat-Nr. : 84173-3-RR

Synonyme

241360D4, Dipeptidyl aminopeptidase III, Dipeptidyl arylamidase III, Dipeptidyl peptidase 3, Dipeptidyl peptidase III

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  WB analysis using 84173-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84173-3-RR (DPP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 84173-3-RR

  WB result of DPP3 antibody (84173-3-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DPP3 transfected HEK-293 cells.

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  Affinity and Kinetic Characterization of 84173-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84173-3-RR against Human DPP3 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84173-3-RR bindet in WB, ELISA DPP3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DPP3 fusion protein Ag1022
• Vollständiger Name: dipeptidyl-peptidase 3
• Berechnetes Molekulargewicht: 83 kDa
• Beobachtetes Molekulargewicht: 73 kDa
• GenBank-Zugangsnummer: BC007221
• Gene symbol: DPP3
• Gene ID (NCBI): 10072
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DPP3 (dipeptidyl-peptidase 3), also known as DPPIII, is one of the dipeptidyl aminopeptidases, that belongs to the peptidase M49 family. DPP3 is a metalloenzyme containing one Zn2+ ion in its active site. Furthermore, It was shown to have a novel zinc-binding motif ( HELLGH). DPP3 has been reported as an important enkephalin-degrading enzyme in the central nervous system, in human neutrophils. It has 3 isoforms produced by alternative splicing. Increased activity of DPP3 is associated with endometrial and ovarian cancers.