DPP4/CD26 Polyklonaler Antikörper

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• KD/KO Validated

DPP4/CD26 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

hamster, human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 10940-1-AP

Synonyme

ADABP, ADCP 2, ADCP2, CD26, dipeptidyl peptidase 4, Dipeptidyl peptidase IV, DPP IV, DPP4, DPPIV, T cell activation antigen CD26, TP103

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Galerie der Validierungsdaten

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  WB analysis of PC-3 using 10940-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10940-1-AP (DPP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 10940-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10940-1-AP (DPP4 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of PC-3 using 10940-1-AP

  Immunofluorescent analysis of PC-3 cells using 10940-1-AP (DPP4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Produktinformation

10940-1-AP bindet in WB, IHC, IF, ELISA DPP4/CD26 und zeigt Reaktivität mit hamster, human

• Getestete Reaktivität: hamster, human
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DPP4/CD26 fusion protein Ag1380
• Vollständiger Name: dipeptidyl-peptidase 4
• Berechnetes Molekulargewicht: 88 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC013329
• Gene symbol: DPP4
• Gene ID (NCBI): 1803
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DPP4 (also known as CD26) is a serine exopeptidase that cleaves X-proline dipeptides from the N terminus of polypeptides. It is an intrinsic membrane glycoprotein anchored into the cell membrane by its N-terminal end. High levels of the enzyme are found in the brush-border membranes of the kidney proximal tubule and of the small intestine, but several other tissues also express the enzyme. The enzyme is present in the fetal colon but disappears at birth. It is ectopically expressed in some human colon adenocarcinomas and human colon cancer cell lines(PMID:1977364).The dimeric 150- 220 kDa DPPIV has been reported to be active and accessible to DFP labeling, but the 110 kDa monomeric DPPIV is not(PMID:9065413). Sometimes traces of the 290 kDa active dimeric form of DPP IV as well as a 55-60 kDa protein appeared in the immunopurified DPP IV preparation. N-terminal amino acid sequence analysis revealed that the 55-60 kDa protein represents a fragment of DPP IV starting at amino acid 28.(PMID:9654125).

Publikationen

Species	Application	Title
human	WB	Oncogene; ApoC1 promotes the metastasis of clear cell renal cell carcinoma via activation of STAT3.; Authors - Yang-Ling Li
rat	WB	Hypertension; Dipeptidyl peptidase IV regulates proliferation of preglomerular vascular smooth muscle and mesangial cells.; Authors - Jackson Edwin K EK
human	WB	Int J Mol Sci; Preclinical Repurposing of Sitagliptin as a Drug Candidate for Colorectal Cancer by Targeting CD24/CTNNB1/SOX4-Centered Signaling Hub; Authors - Jing-Wen Shih
human,rat	WB,IF,IHC	Biochem Pharmacol; Dipeptidyl peptidase-4 inhibition improves endothelial senescence by activating AMPK/SIRT1/Nrf2 signaling pathway.; Authors - Zhihui Chen ; KD Validated
rat	WB	Endocrinology; Dipeptidylpeptidase inhibition is associated with improvement in blood pressure and diastolic function in insulin-resistant male Zucker obese rats.; Authors - Aroor Annayya R AR
rat	IF	J Agric Food Chem; A Low ω-6/ω-3 Ratio High-Fat Diet Improves Rat Metabolism via Purine and Tryptophan Metabolism in the Intestinal Tract, While Reversed by Inulin.; Authors - Yao Xiao