DPP4/CD26 Polyklonaler Antikörper

DPP4/CD26 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 32397-1-AP

Synonyme

CD26, Dipeptidyl peptidase 4, Dipeptidyl peptidase 4 membrane form, Dipeptidyl peptidase 4 soluble form, Dipeptidyl peptidase IV

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  WB analysis using 32397-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32397-1-AP (DPP4/CD26 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 32397-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32397-1-AP (DPP4/CD26 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 32397-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32397-1-AP (DPP4/CD26 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 32397-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 32397-1-AP (DPP4/CD26 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 32397-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 32397-1-AP (DPP4/CD26 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Rattenlebergewebe, Rattenlungengewebe
• Erfolgreiche Detektion in IHC: Rattenlebergewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

32397-1-AP bindet in WB, IHC, ELISA DPP4/CD26 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: dipeptidylpeptidase 4
• Berechnetes Molekulargewicht: 87 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: NM_010074.3
• Gene symbol: Dpp4
• Gene ID (NCBI): 13482
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD26, also known as DPP4 (dipeptidyl peptidase-4), is a 110 kDa type II cell-surface glycoprotein widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial cells, fibroblasts, mesothelium, and endothelial cells of a variety of tissues (PMID:33777580; 26919392). It has ecto-enzyme activity in its extracellular domain and cleaves amino-terminal dipeptides with either L-proline or L-alanine at the penultimate position (PMID: 9553764). CD26 plays roles in diverse biological processes such as immunoregulation, glucose homeostasis, and tumorigenesis (PMID: 33777580). In addition to the 110 kDa monomeric form, a 290 kDa active dimeric form and a 60 kDa fragment of CD26 have also been reported (PMID: 9654125; 7905271).