DPP4/CD26 Monoklonaler Antikörper

DPP4/CD26 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen und mehr (1)

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

2G6D11

Kat-Nr. : 68383-1-Ig

Synonyme

CD26, 2G6D11, ADABP, ADCP 2, ADCP2

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  WB analysis using 68383-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 68383-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HuH-7 using 68383-1-Ig

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 68383-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig kidney using 68383-1-Ig

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit kidney using 68383-1-Ig

  rabbit kidney tissue were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HuH-7 using 68383-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HuH-7 cells using DPP4/CD26 antibody (68383-1-Ig, Clone: 2G6D11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: OS-RC-2 cells, HuH-7-Zellen, humanes Plazenta-Gewebe, HUVEC-Zellen, Hausschwein-Nierengewebe, Kaninchennierengewebe
• Erfolgreiche Detektion in IF/ICC: HuH-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

68383-1-Ig bindet in WB, IF/ICC, ELISA DPP4/CD26 und zeigt Reaktivität mit Hausschwein, human, Kaninchen

• Getestete Reaktivität: Hausschwein, human, Kaninchen
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DPP4/CD26 fusion protein Eg0157
• Vollständiger Name: dipeptidyl-peptidase 4
• Berechnetes Molekulargewicht: 88 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC013329
• Gene symbol: DPP4
• Gene ID (NCBI): 1803
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD26, also known as DPP4 (dipeptidyl peptidase-4), is a 110 kDa type II cell-surface glycoprotein widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial cells, fibroblasts, mesothelium, and endothelial cells of a variety of tissues (PMID:33777580; 26919392). It has ecto-enzyme activity in its extracellular domain and cleaves amino-terminal dipeptides with either L-proline or L-alanine at the penultimate position (PMID: 9553764). CD26 plays roles in diverse biological processes such as immunoregulation, glucose homeostasis, and tumorigenesis (PMID: 33777580).

Publikationen

Species	Application	Title
human	IF	Stem Cell Res Ther; Single-cell sequencing of facial adipose tissue unveils FKBP5 as a therapeutic target for facial infiltrating lipomatosis; Authors - Hongrui Chen
rat	WB	Naunyn Schmiedebergs Arch Pharmacol; Exploring the potential mechanism of atorvastatin in regulating ferroptosis as a treatment for heart failure based on network pharmacology; Authors - Yu-Ting Bai